E2F1抗体;E2F1 Polyclonal Antibod

Description The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor vi r uses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. References 1.Cancer Res. 2010 Dec 1;70(23):9711-20. 2.Mol Cancer Ther. 2010 May;9(5):1265-73. Specification Aliases:RBP3; E2F-1; RBAP1; RBBP3 Entrez GeneID:1869 Swissprot:Q01094 clone:8G9 WB Predicted band size:46.9kDa Host/Isotype:Mouse IgG1 Antibody Type:Primary antibody Storage:Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity:Human,Mouse Immunogen:Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli. Formulation:Purified antibody in PBS with 0.05% sodium azide Application WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FCM:1/200 - 1/400 ELISA:1/10000