DFFA抗体;DFFA Polyclonal Antibod

Description Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. References 1.Nat Commun. 2017 Nov 9;8(1):1392. 2.Cell Rep. 2017 May 2;19(5):1022-1032. Specification Aliases:DFF1; ICAD; DFF-45 Entrez GeneID:1676 Swissprot:O00273 clone:2B10C8 WB Predicted band size:36.5kDa Host/Isotype:Mouse IgG1 Antibody Type:Primary antibody Storage:Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity:Human Immunogen:Purified recombinant fragment of human DFFA (AA: 179-331) expressed in E. Coli. Formulation:Purified antibody in PBS with 0.05% sodium azide Application FCM:1/200 - 1/400 ELISA:1/10000