VIM抗体;VIM Polyclonal Antibody

Description Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments. References 1. Cancer Res. 2003 May 15;63(10):2658-64. 2. Exp Cell Res. 2007 Oct 15;313(17):3718-28. Specification Aliases:VIM Entrez GeneID:7431 Swissprot:P08670 clone:4F2E9 WB Predicted band size:54kDa Host/Isotype:Mouse IgG1 Antibody Type:Primary antibody Storage:Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity:Human,Monkey Immunogen:Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli. Formulation:Ascitic fluid containing 0.03% sodium azide. Application WB:1/500 - 1/2000 IHC:1/200 - 1/1000 ELISA:1/10000