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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Uncharacterized protein C8orf48
- 库存:
50支
- 供应商:
艾普蒂
- 规格:
20μg/50μg
| 规格: | 20μg | 产品价格: | ¥1380.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥2080.0 |
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文献和实验蛋白质、多肽的放射性核素(125I)的标记氯胺T法(ch-T法)
【基本原理】 反应原理不清楚,仅仅是建立在实验基础上,有的学者认为当氯胺T溶于水后释放出次氯酸,将* I- 离子氧化成* I2 、* I+ ,在pH为7.0-8.0条件下与蛋白质酪氨酸残基上邻位氢原子发生置换反应,形成放射性碘标记化合物,用偏重亚硫酸钠终止氧化反应,经过分离纯化,获得纯的标记化合物。 【试剂及材料】 (1)0.05mol/L和0.1mol/L,pH7.4 磷酸缓冲液(PB) (2)氯胺T溶液:用0.05mol/L pH7.4 PB配制成1mg/ml
Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains
scaffolds of much smaller size and high stability. Immunoglobulin-based scaffolds including Fab (∼50 kD), ScFv (∼30 kD), and VH domain (termed domain antibody, dAb) (∼15 kD) have been well established. Recently, a new scaffold based on human IgG1 CH2 domain
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
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