- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
pCMV-SPORT6-RPS3human,NM_001005.5
- 保质期:
3年
- 供应商:
百欧博伟
- 保存条件:
-80
平台编号:bio-114456
启动子:
复制子: ColE1 ori
终止子:
质粒分类: 基因克隆载体
质粒大小:
质粒标签:
原核抗性: 氨苄
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主:
诱导方式:
5'测序引物:
3'测序引物:
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文献和实验encoding enhanced GFP (pEGFP-C1; Clontech) together with 1 µg of either a control plasmid (pMEX), or a pCMV plasmid carrying either the pro-apoptotic adenovirus E1A or the anti-apoptotic adenovirus E1B-19K gene (20 ,21 ) using 10 µl LipofectAMINE Reagent
and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP
of wild-type GFP (1-3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid
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