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- 上海莼试
- CS-01Y66271
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- 详细信息
- 技术资料
- 库存：
23
- 英文名：
Monastrol
- CAS号：
254753-54-3
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
10mM (in 1mL DMSO) 10mg 50mg 500mg 1g
商品属性：
产品描述：
分子式:C14H16N2O3S
分子量:292.35
溶解度:≥ 29.2mg/mL in DMSO
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
IC50: 14 μMMonastrol is a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5. Like other kinesins, Eg5 can drive the movement of microtubules in vitro.In vitro: Previous study found that monastrol did not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol was also reversible rapidly. Chromosomes in monastrol-treated cells frequently had both sister kinetochores attached to microtubules extending to the center of the monoaster. Monastrol also inhibited bipolar spindle formation in Xenopus egg extracts, however, monastrol did not prevent the targeting of Eg5 to the monoastral spindles that form [1].In vivo: Previous study investigated the rat PK and TK of LaSOM 65, a monastrol derivative. Results showed that LaSOM 65 had good bioavailability and linear PK after oral doses. LaSOM 65 distributed consistently in lung and fatty tissues. Other investigated tissues presented smaller penetration ratios. Adverse symptoms were orved only after iv administration, and regressed 3 h after dosing. No statistical differences were found for serum analysis, body weight and relative organ weight, indicating no acute toxicological effects. [2].Clinical trial: Up to now, LaSOM 65 is still in the preclinical development stage.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
公司正在出售的产品：
| 货号 | CS-01Y66271 | 规格 | 10mM (in 1mL DMSO) 10mg 50mg 500mg 1g |
| CAS号 | 254753-54-3 | 分子量 | 292.35 |
| 含量 | >98.00% | 别名 | (±)-Monastrol |
| 分子式 | C14H16N2O3S | 化学名 | ethyl 6-(3-hydroxyphenyl)-2-mercapto-4-methyl-1,6-dihydropyrimidine-5-carboxylate |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C14H16N2O3S
分子量:292.35
溶解度:≥ 29.2mg/mL in DMSO
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
IC50: 14 μMMonastrol is a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5. Like other kinesins, Eg5 can drive the movement of microtubules in vitro.In vitro: Previous study found that monastrol did not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol was also reversible rapidly. Chromosomes in monastrol-treated cells frequently had both sister kinetochores attached to microtubules extending to the center of the monoaster. Monastrol also inhibited bipolar spindle formation in Xenopus egg extracts, however, monastrol did not prevent the targeting of Eg5 to the monoastral spindles that form [1].In vivo: Previous study investigated the rat PK and TK of LaSOM 65, a monastrol derivative. Results showed that LaSOM 65 had good bioavailability and linear PK after oral doses. LaSOM 65 distributed consistently in lung and fatty tissues. Other investigated tissues presented smaller penetration ratios. Adverse symptoms were orved only after iv administration, and regressed 3 h after dosing. No statistical differences were found for serum analysis, body weight and relative organ weight, indicating no acute toxicological effects. [2].Clinical trial: Up to now, LaSOM 65 is still in the preclinical development stage.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
公司正在出售的产品：
| Recombnant Human PRY1, N-H | 磷酸化肝细胞生长因子受体(原癌基因)封闭多肽 |
| 原纤维蛋白2封闭多肽 | DOK1 Antibody Blocking Peptide |
| LRRC19蛋白封闭多肽 | GPBP1L1 Antibody Blocking Peptide |
| α2巨球蛋白样蛋白1封闭多肽 | Ephrin B3 Antibody Blocking Peptide |
| 通用转录因子3C多肽3/TFIIIC102封闭多肽 | KCNQ1 Antibody Blocking Peptide |
| 促性腺激素释放激素受体封闭多肽 | RERG Antibody Blocking Peptide |
| NK细胞抑制性受体2DL4（CD158D）封闭多肽 | phospho-CTNND1 (Tyr228) Antibody Blocking Peptide |
| 血管紧张素Ⅱ-1型受体封闭多肽 | DNA甲基转移酶-3α封闭多肽 |
| D酪氨酸激酶衰减蛋白1封闭多肽 | AGTR1 Antibody Blocking Peptide |
| 血管壁相连蛋白样蛋白1封闭多肽 | KIR2DL4 Antibody Blocking Peptide |
| 钾离子通道蛋白家族KCNQ1封闭多肽 | GnRHR Antibody Blocking Peptide |
| 酪氨酸蛋白激酶B3封闭多肽 | GTF3C3 Antibody Blocking Peptide |
| Ras相关雌激素调节生长抑制蛋白封闭多肽 | MonastrolA2ML1 Antibody Blocking Peptide |
| 磷酸化δ连环蛋白封闭多肽 | LRRC19 Antibody Blocking Peptide |
| Phospho-Met (Tyr1003) Antibody Blocking Peptide | FBN2 Antibody Blocking Peptide |
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Monastrol
￥600 - 3200
