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- 上海莼试
- CS-01Y75380
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- 2025年07月31日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
32
- 英文名：
Rhodamine 123 (chloride)
- CAS号：
62669-70-9
- 供应商：
上海莼试
- 保存条件：
4°C, protect from light
- 规格：
10mg 25mg 100mg
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y75380 | 规格 | 10mg 25mg 100mg |
| CAS号 | 62669-70-9 | 分子量 | 380.8 |
| 含量 | >99.00% | 别名 | R-22420,R-302,RH123 |
| 分子式 | C21H17N2O3 Cl | 化学名 | 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-xanthylium, monochloride |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C21H17N2O3 Cl
分子量:380.8
溶解度:≤10mg/ml in ethanol;10mg/ml in DMSO;10mg/ml in dimethyl formamide;10mg/ml in HCl(0.1M)
储存条件:4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes. These tracer dyes are becoming increasingly used for the examination of membrane transport processes, since they are easy-to-use and cost effective probes for a number of membrane protein transporters, such as P-glycoprotein.In vitro: Rhodamine 123, which was identified as a member of the rhodamine family of flurone dyes, was used to examine membrane transport by the ABCB1 gene product, MDR1. Previous study determined the λmax for excitation and emission for rhodamine 123 in commonly used solvents and extraction buffers, indicating that the fluorescence of rhodamine 123 was highly dependent on the chemical environment. The optimal parameters are 1% methanol in HBSS. In addition, the uptake of rhodamine 123 into cells was via both passive and active processes, and this occurred mainly by OATP1A2-mediated transport. Furthermore, this previous study quantified the intracellular sequestration and metabolism of rhodamine 123, showing that these were both cell line-dependent factors that might influence the interpretation of transport assays [1].
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
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文献和实验【求助】Rhodamine 123 荧光强度和线粒体膜势能问题，求解！
lanzhou3242 我想问一下，到底是 Rhodamine 123黄绿色荧光强度越强，线粒体膜势能越高，还是相反？ 有的说Rhodamine 123进入活细胞的线粒体后显示的荧光是弱的或者是不发光的， 而有的说被活细胞线粒体俘获后显示很强的荧光强度， 我做药物对细胞凋亡的影响，是不是control应该很强，而随着凋亡的增加，其线粒体膜势能降低，所以黄绿色荧光强度更弱？ fis1314
Measurement of Chloride Movement in Neuronal Preparations
, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand?gated chloride movement in vesicular structures called synaptoneurosomes (also referred to as microsacs), in cultured neurons
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
against proteins in the oxidative phosphorylation (OxPhos) system, all of which are discussed below. MitoTracker Probes: Fixable Mitochondrion-Selective Probes Although conventional fluorescent stains for mitochondria, such as rhodamine 123
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