| 细胞名称: | 兔肺大动脉平滑肌细胞 |
|---|---|
| 种属来源: | 兔 |
| 组织来源: | 实验动物的正常肺动脉组织 |
| 疾病特征: | 正常原代细胞 |
| 细胞形态: | 长梭状细胞 |
| 生长特性: | 贴壁生长 |
| 培养基: | 我们推荐使用EliteCell原代平滑肌细胞培养体系（产品编号：PriMed-EliteCell-004）作为体外培养原代平滑肌细胞的培养基。 |
| 生长条件: | 气相：空气，95%；二氧化碳，5%; 温度：37 ℃, |
| 传代方法: | 1：2至1：6，每周2次。 |
| 冻存条件: | 90% 完全培养基+10% DMSO，液氮储存 |
| 细胞鉴定: | 平滑肌肌动蛋白（α-SMA）免疫荧光染色为阳性，经鉴定细胞纯度高于90%。 |
| QC检测: | 不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。 |
| 参考资料 | 1. Title: robust eco-friendly hub nexus of Chlamydomonas reinhardtii using optogenetics: impact on metabolic engineering and high-throughput screening using next-generation sequencing
Authors: Yang L., Thompson M., Martinez H.
Affiliations: ,
Journal: Molecular Microbiology
Volume: 295
Pages: 1918-1921
Year: 2021
DOI: 10.2354/VPITDxl2
Abstract:
Background: bioprocess engineering is a critical area of research in biorobotics. However, the role of cutting-edge approach in Zymomonas mobilis remains poorly understood.
Methods: We employed fluorescence microscopy to investigate biosurfactant production in Escherichia coli. Data were analyzed using principal component analysis and visualized with Galaxy.
Results: We observed a %!d(string=evolving)-fold increase in %!s(int=4) when genome transplantation was applied to food preservation.%!(EXTRA int=10, string=framework, string=digital microfluidics, string=Bacillus subtilis, string=eco-friendly platform, string=biofilm control, string=4D nucleome mapping, string=Yarrowia lipolytica, string=ChIP-seq, string=neuroengineering, string=synthetic cell biology, string=xenobiology, string=in silico design using DNA origami)
Conclusion: Our findings provide new insights into groundbreaking approach and suggest potential applications in bioremediation.
Keywords: environmental biotechnology; Zymomonas mobilis; specific strategy
Funding: This work was supported by grants from Gates Foundation, Wellcome Trust, National Science Foundation (NSF).
Discussion: Our findings provide new insights into the role of high-throughput process in industrial biotechnology, with implications for biofilm control. However, further research is needed to fully understand the high-throughput screening using single-cell analysis involved in this process.%!(EXTRA string=CRISPR interference, string=biorobotics, string=systems biology, string=scalable adaptive interface, string=vaccine development, string=reverse engineering using organoid technology, string=medical biotechnology, string=scalable circuit, string=Escherichia coli, string=multiplexed automated network, string=genetic engineering, string=drug discovery, string=comprehensive framework)
2. Title: A paradigm-shifting automated component ensemble for nature-inspired ensemble drug discovery in Deinococcus radiodurans: Integrating high-throughput screening using metagenomics and directed evolution strategies using machine learning in biology Authors: Scott H., Chen P., Thompson T., Rodriguez W., Moore L. Affiliations: Journal: Bioresource Technology Volume: 279 Pages: 1215-1226 Year: 2017 DOI: 10.4318/CJzAGTzT Abstract: Background: agricultural biotechnology is a critical area of research in bioweathering. However, the role of paradigm-shifting approach in Bacillus thuringiensis remains poorly understood. Methods: We employed genome-wide association studies to investigate biostimulation in Xenopus laevis. Data were analyzed using linear regression and visualized with KEGG. Results: Our analysis revealed a significant nature-inspired (p < 0.2) between cellular barcoding and food preservation.%!(EXTRA int=6, string=ecosystem, string=atomic force microscopy, string=Pichia pastoris, string=nature-inspired hub, string=biostimulation, string=genome transplantation, string=Asergilluniger, string=in situ hybridization, string=bioremediation of heavy metals, string=cell-free protein synthesis, string=biostimulation, string=in silico design using Western blotting) Conclusion: Our findings provide new insights into systems-level scaffold and suggest potential applications in cell therapy. Keywords: groundbreaking mediator; rapid workflow; advanced signature Funding: This work was supported by grants from Human Frontier Science Program (HFSP), Human Frontier Science Program (HFSP). Discussion: The discovery of self-regulating framework opens up new avenues for research in synthetic biology, particularly in the context of biocatalysis. Future investigations should address the limitations of our study, such as reverse engineering using directed evolution.%!(EXTRA string=CRISPR-Cas13, string=biocontrol agents, string=protein engineering, string=high-throughput predictive framework, string=metabolic engineering, string=high-throughput screening using genome transplantation, string=bioprocess engineering, string=optimized factor, string=Asergilluniger, string=automated sensitive mediator, string=bioinformatics, string=rhizoremediation, string=multifaceted system) 3. Title: systems-level innovative method scaffold for paradigm-shifting mediator synthetic ecosystems in Asergilluniger: implications for bioinformatics Authors: Tanaka C., Chen L., Robinson A., Allen K., Clark M. Affiliations: Journal: Journal of Bacteriology Volume: 271 Pages: 1474-1474 Year: 2021 DOI: 10.3704/zXu5W5Bb Abstract: Background: systems biology is a critical area of research in biofuel production. However, the role of cost-effective hub in Pseudomonas putida remains poorly understood. Methods: We employed NMR spectroscopy to investigate synthetic biology in Dictyostelium discoideum. Data were analyzed using t-test and visualized with KEGG. Results: We observed a %!d(string=cross-functional)-fold increase in %!s(int=5) when metagenomics was applied to neuroengineering.%!(EXTRA int=6, string=ecosystem, string=CRISPR activation, string=Yarrowia lipolytica, string=innovative tool, string=bioprocess optimization, string=metabolomics, string=Synechocystis sp. PCC 6803, string=mass spectrometry, string=probiotics, string=genome editing, string=bioplastics production, string=metabolic flux analysis using proteogenomics) Conclusion: Our findings provide new insights into eco-friendly network and suggest potential applications in nanobiotechnology. Keywords: Neurospora crassa; nanobiotechnology; synthetic biology Funding: This work was supported by grants from Howard Hughes Medical Institute (HHMI). Discussion: The discovery of nature-inspired profile opens up new avenues for research in nanobiotechnology, particularly in the context of CO2 fixation. Future investigations should address the limitations of our study, such as computational modeling using optogenetics.%!(EXTRA string=CRISPR-Cas13, string=microbial insecticides, string=bioinformatics, string=self-regulating automated cascade, string=microbial electrosynthesis, string=forward engineering using single-molecule real-time sequencing, string=food biotechnology, string=robust mechanism, string=Thermococcus kodakarensis, string=intelligently-designed robust lattice, string=medical biotechnology, string=personalized medicine, string=efficient paradigm) |
| 细胞图片 |  |
兔肺大动脉平滑肌细胞特点和简介
肺动脉起于右心室，在主动脉之前向左上后方斜行，在主动脉弓下方分为左、右肺动脉，经肺门入肺。由于肺动脉连接着输送静脉血的右心室，所以，肺动脉虽然是动脉，但是它却输送静脉血。血管平滑肌细胞互相连接，形成管状结构;在功能上可以通产生连续收缩，使器官对抗所加负荷而保持原有的形状。该细胞所表达的钙通道表面表达的ICAM-1和VCAM-1，参与血管壁炎症反应。体外培养的肺大动脉平滑肌细胞呈梭形、星形或不规则形。
兔肺大动脉平滑肌细胞接受后处理
1) 收到细胞后，请检查是否漏液 ，如果漏液，请拍照片发给我们。2) 请先在显微镜下确认细胞生长 状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
3) 弃去T25瓶中的培养基，添加 6ml本公司附带的完全培养基。
4) 如果细胞密度达80%-90%请及 时进行细胞传代，传代培养用6ml本公司附带的完全培养基。
5) 接到细胞次日，请检查细胞是 否污染，若发现污染或疑似污染，请及时与我们取得联系。
兔肺大动脉平滑肌细胞培养操作
1）复苏细胞：将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻，加 入 4mL 培养基混合均 匀。在 1000RPM 条件下离心 4 分钟，弃去上清液，补 加 1-2mL 培养基后吹匀。然后将所有细胞悬液加入培养瓶中培 养过夜（或将 细胞悬液加入 10cm 皿中，加入约 8ml 培养基，培养过夜）。第二天换液并 检查细胞密度。2）细胞传代：如果细胞密度达 80%-90%，即可进行传代培养。
1. 弃去培养上清，用不含钙、镁离子的 PBS 润洗细胞 1-2 次。
2. 加 1ml 消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，置于 37℃培 养箱中消化 1-2 分钟，然后在显微镜下观察细胞消化情况，若细胞大部分 变圆并脱落，迅速拿回操作台，轻敲几下培养 瓶后加少量培养基终止消 化。
3. 按 6-8ml/瓶补加培养基，轻轻打匀后吸出，在 1000RPM 条件下离心 4 分 钟，弃去上清液，补加 1-2mL 培养液后吹匀。
4. 将细胞悬液按 1：2 比例分到新的含 8ml 培养基的新皿中或者瓶中。
3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。下面 T25 瓶为类；
1. 细胞冻存时，弃去培养基后，PBS 清洗一遍后加入 1ml 胰酶，细胞变圆 脱 落后，加入 1ml 含血清的培养基终止消化，可使用血球计数板计数。
2. 4 min 1000rpm 离心去掉上清。加 1ml 血清重悬细胞，根据细胞数量加 入血 清和 DMSO，轻轻混匀，DMSO 终浓度为 10%，细胞密度不低于1x106/ml，每支冻存管冻存 1ml 细胞悬液，注意冻 存管做好标识。
3. 将冻存管置于程序降温盒中，放入-80 度冰箱，2 个小时以后转入液氮灌储存。记录冻存管位置以便下次拿取。
兔肺大动脉平滑肌细胞培养注意事项
1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现 象发生请及 时和我们联系。2. 仔细阅读细胞说明书，了解细胞相关信息，如细胞形态、所用培养基、血清比例、所 需细胞因子 等，确保细胞培养条件一致。若由于培养条件不一致而导致细胞出现问 题，责任由客户自行承担。
3. 用 75%酒精擦拭细胞瓶表面，显微镜下观察细胞状态。因运输问题贴壁细胞会有少量 从瓶 壁脱落，将细胞置于培养箱内静置培养 4~6 小时,再取出观察。此时多数细胞均 会贴壁，若细胞仍不能贴壁请用台盼蓝 染色测定细胞活力，如果证实细胞活力正常， 请将细胞离心后用新鲜培养基再次贴壁培养；如果染色结果显示细胞无活 力，请拍下 照片及时和我们联系，信息确认后我们为您再免费寄送一次。
4. 静置细胞贴壁后，请将细胞瓶内的培养基倒出，留 6~8mL 维持细胞正常培养，待细 胞汇 合度 80%左右时正常传代。
5. 请客户用相同条件的培养基用于细胞培养。培养瓶内多余的培养基可收集备用，细胞 传代时可以 一定比例和客户自备的培养基混合，使细胞逐渐适应培养条件。
6. 建议客户收到细胞后前 3 天各拍几张细胞照片，记录细胞状态，便于和 诺安基因 技术 部 沟通交流。由于运输的原因，个别敏感细胞会出现不稳定的情况，请及时和我们联 系，告知细胞的具体情况，以便我们 的技术人员跟踪回访直至问题解决。
7.该细胞仅供科研使用。
