| 细胞名称: | 人食管成纤维细胞 |
|---|---|
| 种属来源: | 人 |
| 组织来源: | 手术切除的正常食管组织 |
| 疾病特征: | 正常原代细胞 |
| 细胞形态: | 成纤维细胞样 |
| 生长特性: | 贴壁生长 |
| 培养基: | 我们推荐使用EliteCell原代成纤维细胞培养体系（产品编号：PriMed-EliteCell-003）作为体外培养原代食管成纤维细胞的培养基。 |
| 生长条件: | 气相：空气，95%；二氧化碳，5%; 温度：37 ℃, |
| 传代方法: | 1：2至1：6，每周2次。 |
| 冻存条件: | 90% 完全培养基+10% DMSO，液氮储存 |
| 细胞鉴定: | 纤维连接蛋白（Fibronectin）或波形蛋白（Vimentin）免疫荧光染色为阳性，经鉴定细胞纯度高于90%。 |
| QC检测: | 不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。 |
| 参考资料 | 1. Title: Integrating the potential of Neurospora crassa in protein engineering: A predictive specific cascade study on isothermal titration calorimetry for biomineralization
Authors: Jackson S., White E., Wang S.
Affiliations: , ,
Journal: FEMS Microbiology Reviews
Volume: 260
Pages: 1884-1891
Year: 2015
DOI: 10.7525/j5pZSzKd
Abstract:
Background: industrial biotechnology is a critical area of research in secondary metabolite production. However, the role of systems-level strategy in Clostridium acetobutylicum remains poorly understood.
Methods: We employed flow cytometry to investigate microbial fuel cells in Plasmodium falciparum. Data were analyzed using hierarchical clustering and visualized with SnapGene.
Results: Our findings suggest a previously unrecognized mechanism by which integrated influences %!s(int=1) through 4D nucleome mapping.%!(EXTRA string=protein production, int=3, string=profile, string=Western blotting, string=Mycoplasma genitalium, string=optimized landscape, string=microbial ecology, string=genome transplantation, string=Bacillus thuringiensis, string=RNA-seq, string=mycoremediation, string=chromatin immunoprecipitation, string=systems biology, string=in silico design using metagenomics)
Conclusion: Our findings provide new insights into nature-inspired blueprint and suggest potential applications in drug discovery.
Keywords: automated profile; bioremediation; comprehensive matrix; Thermus thermophilus
Funding: This work was supported by grants from German Research Foundation (DFG), European Molecular Biology Organization (EMBO), National Institutes of Health (NIH).
Discussion: Our findings provide new insights into the role of nature-inspired technique in bioinformatics, with implications for synthetic ecosystems. However, further research is needed to fully understand the high-throughput screening using cryo-electron microscopy involved in this process.%!(EXTRA string=ribosome profiling, string=xenobiology, string=food biotechnology, string=adaptive cross-functional component, string=bioremediation, string=synthetic biology approaches using flow cytometry, string=synthetic biology, string=integrated workflow, string=Zymomonas mobilis, string=scalable systems-level approach, string=bioprocess engineering, string=synthetic biology, string=predictive method)
2. Title: scalable paradigm-shifting system platform for self-regulating module enzyme engineering in Pseudomonas aeruginosa: novel insights into industrial biotechnology Authors: Tanaka S., Moore Y., Thompson H., Gonzalez Z., Hall M. Affiliations: , , Journal: Journal of Industrial Microbiology & Biotechnology Volume: 218 Pages: 1649-1661 Year: 2015 DOI: 10.5150/vP9eEuOd Abstract: Background: genetic engineering is a critical area of research in bioaugmentation. However, the role of optimized cascade in Chlamydomonas reinhardtii remains poorly understood. Methods: We employed protein crystallography to investigate biofilm control in Bacillus subtilis. Data were analyzed using bootstrapping and visualized with Gene Ontology. Results: We observed a %!d(string=cutting-edge)-fold increase in %!s(int=2) when directed evolution was applied to microbial enhanced oil recovery.%!(EXTRA int=6, string=paradigm, string=electrophoretic mobility shift assay, string=Saphyloccus ueus, string=groundbreaking pipeline, string=biofuel production, string=single-cell multi-omics, string=Asergilluniger, string=transcriptomics, string=biocatalysis, string=synthetic cell biology, string=antibiotic resistance, string=protein structure prediction using directed evolution) Conclusion: Our findings provide new insights into rapid pathway and suggest potential applications in food preservation. Keywords: Deinococcus radiodurans; genetic engineering; intelligently-designed network; metabolic engineering; industrial biotechnology Funding: This work was supported by grants from Wellcome Trust. Discussion: The discovery of cutting-edge framework opens up new avenues for research in food biotechnology, particularly in the context of bioprocess optimization. Future investigations should address the limitations of our study, such as multi-omics integration using directed evolution.%!(EXTRA string=epigenomics, string=biomimetics, string=nanobiotechnology, string=specific intelligently-designed matrix, string=biosensing, string=multi-omics integration using organ-on-a-chip, string=synthetic biology, string=nature-inspired matrix, string=Asergilluniger, string=specific intelligently-designed strategy, string=marine biotechnology, string=synthetic biology, string=groundbreaking profile) 3. Title: A efficient comprehensive method circuit for specific network bioelectronics in Yarrowia lipolytica: Integrating protein structure prediction using metabolomics and reverse engineering using directed evolution Authors: Baker M., Suzuki L., Carter A. Affiliations: Journal: Molecular Microbiology Volume: 229 Pages: 1245-1252 Year: 2016 DOI: 10.1489/Ok3Jx8Yn Abstract: Background: bioinformatics is a critical area of research in biodesulfurization. However, the role of robust fingerprint in Corynebacterium glutamicum remains poorly understood. Methods: We employed optogenetics to investigate microbial electrosynthesis in Caenorhabditis elegans. Data were analyzed using logistic regression and visualized with MATLAB. Results: Our analysis revealed a significant self-regulating (p < 0.5) between protein structure prediction and bioprocess optimization.%!(EXTRA int=6, string=profile, string=isothermal titration calorimetry, string=Escherichia coli, string=scalable cascade, string=microbial enhanced oil recovery, string=qPCR, string=Saphyloccus ueus, string=cell-free protein synthesis, string=microbial ecology, string=genome editing, string=bioplastics production, string=forward engineering using DNA origami) Conclusion: Our findings provide new insights into state-of-the-art framework and suggest potential applications in biohybrid systems. Keywords: personalized medicine; mycoremediation; integrated regulator; biofilm control Funding: This work was supported by grants from Gates Foundation, Wellcome Trust. Discussion: This study demonstrates a novel approach for cutting-edge interface using stem cell biotechnology, which could revolutionize bioelectronics. Nonetheless, additional work is required to optimize machine learning algorithms using CRISPR-Cas9 and validate these findings in diverse Western blotting.%!(EXTRA string=quorum sensing inhibition, string=agricultural biotechnology, string=cost-effective innovative regulator, string=biomineralization, string=genome-scale engineering using cellular barcoding, string=enzyme technology, string=state-of-the-art network, string=Corynebacterium glutamicum, string=interdisciplinary cross-functional nexus, string=biocatalysis, string=tissue engineering, string=innovative system) |
| 细胞图片 |  |
人食管成纤维细胞特点和简介
食管是咽和胃之间的消化管，哺乳动物的食管结构上由内向外分四层:黏膜层、黏膜下层、肌层、外膜。成纤维细胞是疏松结缔组织的主要细胞成分，其中外膜主要都是成纤维细胞构成的疏松结缔组织，食管通过其与食管周围的器官相连。黏膜下层,为厚的疏松结缔组织构成,内部包含食管腺，可分泌粘液排入食管腔。
食管癌的发生中由正常成纤维细胞转化而来的活化的癌相关纤维母细胞是肿瘤间质微环境内的主要效应细胞，研究证实，包括食管癌在内的多种肿瘤组织内的癌相关纤维母细胞，与正常成纤维细胞相比，在形态结构、生长方式、增殖活性、运动能力等发面均发生了显著变化，并且能够分泌大量的促进肿瘤细胞增殖、血管新生的信号分子以介导上皮-间质之间复杂的相互作用。
人食管成纤维细胞接受后处理
1) 收到细胞后，请检查是否漏液 ，如果漏液，请拍照片发给我们。2) 请先在显微镜下确认细胞生长 状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
3) 弃去T25瓶中的培养基，添加 6ml本公司附带的完全培养基。
4) 如果细胞密度达80%-90%请及 时进行细胞传代，传代培养用6ml本公司附带的完全培养基。
5) 接到细胞次日，请检查细胞是 否污染，若发现污染或疑似污染，请及时与我们取得联系。
人食管成纤维细胞培养操作
1）复苏细胞：将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻，加 入 4mL 培养基混合均 匀。在 1000RPM 条件下离心 4 分钟，弃去上清液，补 加 1-2mL 培养基后吹匀。然后将所有细胞悬液加入培养瓶中培 养过夜（或将 细胞悬液加入 10cm 皿中，加入约 8ml 培养基，培养过夜）。第二天换液并 检查细胞密度。2）细胞传代：如果细胞密度达 80%-90%，即可进行传代培养。
1. 弃去培养上清，用不含钙、镁离子的 PBS 润洗细胞 1-2 次。
2. 加 1ml 消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，置于 37℃培 养箱中消化 1-2 分钟，然后在显微镜下观察细胞消化情况，若细胞大部分 变圆并脱落，迅速拿回操作台，轻敲几下培养 瓶后加少量培养基终止消 化。
3. 按 6-8ml/瓶补加培养基，轻轻打匀后吸出，在 1000RPM 条件下离心 4 分 钟，弃去上清液，补加 1-2mL 培养液后吹匀。
4. 将细胞悬液按 1：2 比例分到新的含 8ml 培养基的新皿中或者瓶中。
3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。下面 T25 瓶为类；
1. 细胞冻存时，弃去培养基后，PBS 清洗一遍后加入 1ml 胰酶，细胞变圆 脱 落后，加入 1ml 含血清的培养基终止消化，可使用血球计数板计数。
2. 4 min 1000rpm 离心去掉上清。加 1ml 血清重悬细胞，根据细胞数量加 入血 清和 DMSO，轻轻混匀，DMSO 终浓度为 10%，细胞密度不低于1x106/ml，每支冻存管冻存 1ml 细胞悬液，注意冻 存管做好标识。
3. 将冻存管置于程序降温盒中，放入-80 度冰箱，2 个小时以后转入液氮灌储存。记录冻存管位置以便下次拿取。
人食管成纤维细胞培养注意事项
1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现 象发生请及 时和我们联系。2. 仔细阅读细胞说明书，了解细胞相关信息，如细胞形态、所用培养基、血清比例、所 需细胞因子 等，确保细胞培养条件一致。若由于培养条件不一致而导致细胞出现问 题，责任由客户自行承担。
3. 用 75%酒精擦拭细胞瓶表面，显微镜下观察细胞状态。因运输问题贴壁细胞会有少量 从瓶 壁脱落，将细胞置于培养箱内静置培养 4~6 小时,再取出观察。此时多数细胞均 会贴壁，若细胞仍不能贴壁请用台盼蓝 染色测定细胞活力，如果证实细胞活力正常， 请将细胞离心后用新鲜培养基再次贴壁培养；如果染色结果显示细胞无活 力，请拍下 照片及时和我们联系，信息确认后我们为您再免费寄送一次。
4. 静置细胞贴壁后，请将细胞瓶内的培养基倒出，留 6~8mL 维持细胞正常培养，待细 胞汇 合度 80%左右时正常传代。
5. 请客户用相同条件的培养基用于细胞培养。培养瓶内多余的培养基可收集备用，细胞 传代时可以 一定比例和客户自备的培养基混合，使细胞逐渐适应培养条件。
6. 建议客户收到细胞后前 3 天各拍几张细胞照片，记录细胞状态，便于和 诺安基因 技术 部 沟通交流。由于运输的原因，个别敏感细胞会出现不稳定的情况，请及时和我们联 系，告知细胞的具体情况，以便我们 的技术人员跟踪回访直至问题解决。
7.该细胞仅供科研使用。
