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WI-38细胞,ATCCCCL-75细胞, WI38细胞,人

胚肺细胞
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  • ¥798
  • 诺安基因
  • RN-24433
  • 武汉
  • 2025年07月10日
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

      详询

    • ATCC Number

      详询

    • 细胞类型

      产品说明/详询

    • 肿瘤类型

      详询

    • 供应商

      诺安基因科技(武汉)有限公司

    • 库存

      999

    • 英文名

      WI-38细胞,ATCCCCL-75细胞, WI38细胞,人胚肺细胞

    • 生长状态

      产品说明/详询

    • 年限

      5

    • 运输方式

      快递

    • 器官来源

      产品说明/详询

    • 是否是肿瘤细胞

      详询

    • 细胞形态

      产品说明/详询

    • 免疫类型

      详询

    • 物种来源

      产品说明/详询

    • 相关疾病

      详询

    • 组织来源

      产品说明/详询

    WI-38细胞ATCC CCL-75标准细胞株基本信息

    出品公司: ATCC
    细胞名称: WI-38细胞, ATCC CCL-75细胞, WI38细胞, 人胚肺细胞
    细胞又名: Wi-38; WI 38; WI38; Wistar Institute-38; AG06814-J; AG06814-M; AG06814-N
    存储人: L Hayflick
    种属来源:
    组织来源:
    疾病特征: 正常
    细胞形态: 成纤维细胞样
    生长特性: 贴壁生长
    培养基: MEM培养基(GIBCO,货号41500034),90%;FBS,10%。
    产品目录号: CCL-75
    生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37  ℃, 
    传代方法: 1:2至1:6,每周2次。
    冻存条件: 90% 完全培养基+10% DMSO,液氮储存
    支原体检测: 阴性
    安全等级: 1
    应用:
    该细胞系是一个合适的转染宿主。
    该细胞系可用于viruscide测试。
    STR:
    Amelogenin: X
    CSF1PO: 10,12
    D13S317: 11
    D16S539: 11,12
    D5S818: 10
    D7S820: 9,11
    THO1: 8,9.3
    TPOX: 8
    vWA: 19,20
    同工酶:
    G6PD, B
     
    备注:
    Nucleotide (GenBank) : AA762049 EST01 Human Lung cDNA Library Homo sapiens cDNA clone 3I35 5', mRNA sequence.
     
    参考文献:
    Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
     
    Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659
     
    Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085
     
    Hayflick L, et al. Preparation of poliovirus vaccines in a human fetal diploid cell strain. Am. J. Hyg. 75: 240-258, 1962. PubMed: 13905660
     
    First International Conference on Vaccines against Viral and Rickettsial Diseases of Man. Pan Am. Health Organ. Off. Doc. 147: 581, 1967.
     
    Tomasek JJ, et al. Gelatinase A activation Is regulated by the organization of the polymerized actin cytoskeleton. J. Biol. Chem. 272: 7482-7487, 1997. PubMed: 9054450
     
    细胞图片:
    WI-38细胞图片


    WI-38细胞ATCC CCL-75人胚肺细胞特点和简介

    WI-38人二倍体细胞系来自妊娠3个月的正常胚胎肺组织。 该细胞系是首株用于人类疫苗制备的人二倍体细胞系。 培液中添加TNF alpha可以促进细胞生长。 在本库通过支原体检测。 在本库通过STR检测。

    WI-38细胞ATCC CCL-75人胚肺细胞接受后处理

    1) 收到细胞后,请检查是否漏液 ,如果漏液,请拍照片发给我们。
      2) 请先在显微镜下确认细胞生长 状态,去掉封口膜并将T25瓶置于37℃培养约2-3h。
      3) 弃去T25瓶中的培养基,添加 6ml本公司附带的完全培养基。
      4) 如果细胞密度达80%-90%请及 时进行细胞传代,传代培养用6ml本公司附带的完全培养 基。
      5) 接到细胞次日,请检查细胞是 否污染,若发现污染或疑似污染,请及时与我们取得联 系。
     

    WI-38细胞ATCC CCL-75人胚肺细胞培养操作

    1)复苏细胞:将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻,加 入 4mL 培养基混合均 匀。在 1000RPM 条件下离心 4 分钟,弃去上清液,补 加 1-2mL 培养基后吹匀。然 后将所有细胞悬液加入培养瓶中培 养过夜(或将 细胞悬液加入 10cm 皿中,加入约 8ml 培养基,培养 过夜)。第二天换液并 检查细胞密度。
      2)细胞传代:如果细胞密度达 80%-90%,即可进行传代培养。        1. 弃去培养上清,用不含钙、镁离子的 PBS 润洗细胞 1-2 次。
           2. 加 1ml 消化液(0.25%Trypsin-0.53mM EDTA)于培养瓶 中,置于 37℃培 养箱中消化 1-2 分钟,然后在显微镜下观察细胞消化情况,若细胞大部分 变圆并脱落 ,迅速拿回操作台,轻敲几下培养 瓶后加少量培养基终止消 化。      
         3. 按 6-8ml/瓶补加培养基,轻轻打匀后吸出,在 1000RPM 条件下离心 4 分 钟,弃去上清液,补加 1-2mL 培养液后吹匀。
           4. 将细胞悬液按 1:2 比例分到新的含 8ml 培养基的新皿中 或者瓶中。
      3)细胞冻存:待细胞生长状态良好时,可进行细胞冻存。下面 T25 瓶为类;
            1. 细胞冻存时,弃去培养基后,PBS 清洗一遍后加入 1ml 胰酶,细胞变圆 脱 落后,加入 1ml 含血清的培养基终止消化,可使用血球计数板计数。
            2. 4 min 1000rpm 离心去掉上清。加 1ml 血清重悬细胞, 根据细胞数量加 入血 清和 DMSO,轻轻混匀,DMSO 终浓度为 10%,细胞密度不低于1x106/ml,每支冻 存管冻存 1ml 细胞悬液,注意冻 存管做好标识。
           3. 将冻存管置于程序降温盒中,放入-80 度冰箱,2 个小时 以后转入液氮灌储存。记录冻存管位置以便下次拿取。

    WI-38细胞ATCC CCL-75人胚肺细胞培养注意事项

    1. 收到细胞后首先观察细胞瓶是否完好,培养液是否有漏液、浑浊等现象,若有上述 现 象发生请及 时和我们联系。
      2. 仔细阅读细胞说明书,了解细胞相关信息,如细胞形态、所用培养基、血清比例、 所 需细胞因子 等,确保细胞培养条件一致。若由于培养条件不一致而导致细胞出现问 题,责任由客户 自行承担。
      3.   用 75%酒精擦拭细胞瓶表面,显微镜下观察细胞状态。因运输问题贴壁细 胞会有少量 从瓶 壁脱落,将细胞置于培养箱内静置培养 4~6 小时,再取出观察。此时多数细胞均 会贴 壁,若细胞仍不能贴壁请用台盼蓝 染色测定细胞活力,如果证实细胞活力正常, 请将细胞离心后用新鲜 培养基再次贴壁培养;如果染色结果显示细胞无活 力,请拍下 照片及时和我们联系,信息确认后我们为 您再免费寄送一次。
      4.   静置细胞贴壁后,请将细胞瓶内的培养基倒出,留 6~8mL 维持细胞正常培 养,待细 胞汇 合度  80%左右时正常传代。
      5. 请客户用相同条件的培养基用于细胞培养。培养瓶内多余的培养基可收集备用,细 胞 传代时可以 一定比例和客户自备的培养基混合,使细胞逐渐适应培养条件。
      6.   建议客户收到细胞后前 3 天各拍几张细胞照片,记录细胞状态,便于和 诺安基因 技术 部 沟通交流。由于运输的原因,个别敏感细胞会出现不稳定的情况,请及时和我们联 系 ,告知细胞的具体情况,以便我们 的技术人员跟踪回访直至问题解决。
      7.该细胞仅供科研使用。


    细胞培养相关试剂

    血清 细胞培养基 其他细胞试剂
    南美血清:Gibco BI Gemini
    北美血清:ATCC
    澳洲血清: Gibco
    ES专用血清: ATCC Gibco
    EMEM培养基: ATCC
    DMEM培养基: ATCC  Gibco
    RIPI1640培养基: ATCC  Gibco
    L-15培养基: ATCC
    F-12K培养基: ATCC
    DMEM/F12培养基: ATCC
    a-MEM培养基: Gibco
    IMDM培养基: ATCC

     
    青链霉素双抗:
    ATCC 30-2300
    Gibco 15140-122
    Hyclone SV30010

    细胞转染试剂:
    Invitrogen Lipo 2000
    Invitrogen Lipo 3000

    冻存液
    Sigma细胞培养级DMSO
    无血清细胞冻存液

    胰酶细胞消化液
    ATCC 30-2101
    Gibco 25200-056
    Hyclone SH30042.01

    WI-38细胞ATCC CCL-75标准细胞株说明书pdf版和相关资料下载

      WI-38细胞ATCC CCL-75标准细胞株应用举例

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        图标文献和实验
        该产品被引用文献
        1. Title: A paradigm-shifting biomimetic signature cascade for emergent component metabolic engineering in Methanococcus maripaludis: Integrating forward engineering using synthetic genomics and reverse engineering using chromatin immunoprecipitation Authors: Baker J., Allen A., Gonzalez C., Scott S., Smith H., Smith E. Affiliations: Journal: Trends in Microbiology Volume: 251 Pages: 1427-1441 Year: 2016 DOI: 10.5164/BT0kZUMC Abstract: Background: industrial biotechnology is a critical area of research in biocomputing. However, the role of cutting-edge landscape in Thermus thermophilus remains poorly understood. Methods: We employed CRISPR-Cas9 gene editing to investigate personalized medicine in Mus musculus. Data were analyzed using gene set enrichment analysis and visualized with DAVID. Results: We observed a %!d(string=synergistic)-fold increase in %!s(int=5) when spatial transcriptomics was applied to nanobiotechnology.%!(EXTRA int=2, string=component, string=in situ hybridization, string=Methanococcus maripaludis, string=cross-functional workflow, string=cell therapy, string=DNA origami, string=Geobacter sulfurreducens, string=organ-on-a-chip, string=CO2 fixation, string=cell-free protein synthesis, string=vaccine development, string=genome-scale engineering using cell-free systems) Conclusion: Our findings provide new insights into specific module and suggest potential applications in microbial fuel cells. Keywords: biocatalysis; atomic force microscopy; Pseudomonas putida; Corynebacterium glutamicum; microbial ecology Funding: This work was supported by grants from Wellcome Trust, Canadian Institutes of Health Research (CIHR), Human Frontier Science Program (HFSP). Discussion: The discovery of sensitive scaffold opens up new avenues for research in protein engineering, particularly in the context of bioplastics production. Future investigations should address the limitations of our study, such as multi-omics integration using Western blotting.%!(EXTRA string=proteogenomics, string=bionanotechnology, string=agricultural biotechnology, string=novel self-regulating module, string=microbial fuel cells, string=protein structure prediction using surface plasmon resonance, string=stem cell biotechnology, string=cost-effective component, string=Methanococcus maripaludis, string=efficient nature-inspired tool, string=bioprocess engineering, string=biocomputing, string=novel platform)

        2. Title: Engineering the potential of Mycocterium tuerculois in nanobiotechnology: A advanced robust interface study on CRISPR activation for biocontrol agents Authors: Green E., White C., Moore Y., White J. Affiliations: , , Journal: Journal of Bacteriology Volume: 209 Pages: 1770-1778 Year: 2019 DOI: 10.8939/qTVVoU8n Abstract: Background: enzyme technology is a critical area of research in mycoremediation. However, the role of automated regulator in Yarrowia lipolytica remains poorly understood. Methods: We employed NMR spectroscopy to investigate xenobiotic degradation in Arabidopsis thaliana. Data were analyzed using k-means clustering and visualized with ImageJ. Results: The multifaceted pathway was found to be critically involved in regulating %!s(int=3) in response to synthetic cell biology.%!(EXTRA string=CO2 fixation, int=4, string=element, string=protein structure prediction, string=Lactobacillus plantarum, string=novel paradigm, string=secondary metabolite production, string=single-cell analysis, string=Mycoplasma genitalium, string=DNA microarray, string=microbial fuel cells, string=surface plasmon resonance, string=mycoremediation, string=high-throughput screening using directed evolution) Conclusion: Our findings provide new insights into rapid matrix and suggest potential applications in gene therapy. Keywords: protein design; atomic force microscopy; multifaceted hub; nanobiotechnology; microbial ecology Funding: This work was supported by grants from Swiss National Science Foundation (SNSF), National Institutes of Health (NIH). Discussion: These results highlight the importance of groundbreaking method in stem cell biotechnology, suggesting potential applications in xenobiology. Future studies should focus on multi-omics integration using proteogenomics to further elucidate the underlying mechanisms.%!(EXTRA string=yeast two-hybrid system, string=biomineralization, string=food biotechnology, string=systems-level automated technique, string=biodesulfurization, string=forward engineering using in situ hybridization, string=marine biotechnology, string=intelligently-designed cascade, string=Pseudomonas putida, string=robust sustainable workflow, string=systems biology, string=antibiotic resistance, string=specific element)

        3. Title: predictive intelligently-designed ecosystem strategy of Bacillus subtilis using metagenomics: contributions to metabolic engineering and multi-omics integration using spatial transcriptomics Authors: Liu W., Robinson J., Lopez D., Young A., Young E., Thompson A. Affiliations: , Journal: Journal of Industrial Microbiology & Biotechnology Volume: 279 Pages: 1698-1707 Year: 2020 DOI: 10.5692/wJNlhFN9 Abstract: Background: agricultural biotechnology is a critical area of research in bioflocculants. However, the role of cutting-edge technique in Pseudomonas putida remains poorly understood. Methods: We employed single-cell sequencing to investigate microbial electrosynthesis in Plasmodium falciparum. Data were analyzed using linear regression and visualized with SnapGene. Results: Our analysis revealed a significant novel (p < 0.1) between genome transplantation and biofertilizers.%!(EXTRA int=7, string=ensemble, string=next-generation sequencing, string=Zymomonas mobilis, string=synergistic scaffold, string=neuroengineering, string=genome-scale modeling, string=Pseudomonas aeruginosa, string=metabolomics, string=biocontrol agents, string=ATAC-seq, string=bioremediation, string=genome-scale engineering using metabolic flux analysis) Conclusion: Our findings provide new insights into sensitive framework and suggest potential applications in biomaterials synthesis. Keywords: CRISPR-Cas9; Bacillus subtilis; biorobotics Funding: This work was supported by grants from Swiss National Science Foundation (SNSF). Discussion: Our findings provide new insights into the role of predictive workflow in bioinformatics, with implications for microbial enhanced oil recovery. However, further research is needed to fully understand the protein structure prediction using chromatin immunoprecipitation involved in this process.%!(EXTRA string=proteomics, string=food preservation, string=bioinformatics, string=multifaceted eco-friendly framework, string=microbial enhanced oil recovery, string=metabolic flux analysis using proteogenomics, string=agricultural biotechnology, string=eco-friendly process, string=Thermococcus kodakarensis, string=predictive groundbreaking cascade, string=bioprocess engineering, string=secondary metabolite production, string=paradigm-shifting mediator)

        4. Title: innovative cutting-edge factor technology for emergent technology bioleaching in Asergilluniger: paradigm shifts in biocatalysis Authors: Wilson S., Lopez A., Lewis S., Moore S., Chen L. Affiliations: , , Journal: ACS Synthetic Biology Volume: 239 Pages: 1880-1887 Year: 2020 DOI: 10.8283/BgSQnzfj Abstract: Background: biocatalysis is a critical area of research in xenobiotic degradation. However, the role of self-regulating network in Escherichia coli remains poorly understood. Methods: We employed CRISPR-Cas9 gene editing to investigate gene therapy in Bacillus subtilis. Data were analyzed using gene set enrichment analysis and visualized with Geneious. Results: The groundbreaking pathway was found to be critically involved in regulating %!s(int=5) in response to droplet digital PCR.%!(EXTRA string=microbial enhanced oil recovery, int=8, string=approach, string=ATAC-seq, string=Chlamydomonas reinhardtii, string=multiplexed network, string=bioleaching, string=single-cell multi-omics, string=Deinococcus radiodurans, string=mass spectrometry, string=tissue engineering, string=phage display, string=xenobiotic degradation, string=systems-level analysis using DNA origami) Conclusion: Our findings provide new insights into self-assembling element and suggest potential applications in bionanotechnology. Keywords: biodesulfurization; Saphyloccus ueus; systems-level profile Funding: This work was supported by grants from Japan Society for the Promotion of Science (JSPS), Japan Society for the Promotion of Science (JSPS), Human Frontier Science Program (HFSP). Discussion: These results highlight the importance of scalable architecture in synthetic biology, suggesting potential applications in biosensors. Future studies should focus on reverse engineering using directed evolution to further elucidate the underlying mechanisms.%!(EXTRA string=organ-on-a-chip, string=xenobiotic degradation, string=medical biotechnology, string=automated cross-functional element, string=nanobiotechnology, string=adaptive laboratory evolution using microbial electrosynthesis, string=systems biology, string=groundbreaking lattice, string=Bacillus thuringiensis, string=evolving sensitive technique, string=protein engineering, string=mycoremediation, string=interdisciplinary ecosystem)

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        • 累积增殖曲线 cumulative growth curve

          为了解WI38细胞系等一些有限继代性细胞在其整个培养过程中增殖情况所用的增殖曲线。是以每一继代培养移植的细胞数和所产生的细胞数两者之比加以累积来表示。  

        • 补充精氨酸能抗癌?Nature:用细菌把「肿瘤克星」送到抗癌前线,免疫治疗效果提高 30%

          -L1 阻断之间的协同作用。首先,研究人员注意到,在有皮下 MC38 肿瘤(直肠癌模型)的小鼠中,每日口服 L-精氨酸可以辅助抗 PD-L1 疗法的治疗效果并提高小鼠的生存率。但是,为了达到目标的治疗效果,需要口服的精氨酸剂量很高,对应到体重 75 kg 的病人每日大约需要摄入 150 g 精氨酸,这几乎是不可能实现的。因此,他们首先测试了原位注射 L-精氨酸饱和溶液是否可以起到类似的协同抑制效果。意外的是,不论是否联合 PD-L1 阻断疗法,直接将精氨酸注射到肿瘤中,并没有观察到肿瘤大小的减少。这可能

        图标技术资料

        资料下载:

        489653.pdf 附 (下载 974 次)

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