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- 上海莼试
- CS-01Y68940
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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
33
- 英文名：
MJ33 (lithium salt)
- CAS号：
1007476-63-2
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
1mg 5mg 10mg 25mg
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y68940 | 规格 | 1mg 5mg 10mg 25mg |
| CAS号 | 1007476-63-2 | 分子量 | 498.5 |
| 含量 | >98.00% | 别名 | |
| 分子式 | C22H43F3O6P? Li | 化学名 | mono[1-?[(hexadecyloxy)?methyl]trifluoroethoxy)?ethyl] monomethyl ester phosphoric acid, monolithium salt |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C22H43F3O6P? Li
分子量:498.5
溶解度:≤2mg/ml in ethanol;0.25mg/ml in DMSO;0.5mg/ml in dimethyl formamide
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
MJ33 is an inhibitor of the acidic, calcium-independent (ai)PLA2 activity of Prdx6.Peroxiredoxin-6 (Prdx6), a bifunctional enzyme, has both non-selenium glutathione peroxidase and phospholipase A2 (PLA2) activities. The PLA2 activity of Prdx6 is calcium-independent, functions optimally in acidic conditions, and facilitates the intracellular processing of surfactant lipids, such as dipalmitoylphosphatidylcholine.In vitro: MJ33 was found to be specifically inhibit the aiPLA2 activity of the protein. Moreover, the Ca2+-independent PLA2 activity of phosphorylated rat Prdx6 could be abolished by the treatment of either MJ33 or surfactant protein A (SP-A), known inhibitors of aiPLA2 activity. Further supporting the results with intact cells, recombinant Prdx6 was phosphorylated in vitro by ERK and p38, but not by JNK. Phosphorylation in vitro led to a great increase in PLA2 activity that was Ca2+-independent and ould be inhibited by both MJ33 and by SP-A, which was similar to native lung enzyme [1].In vivo: A previous study evaluated the effect of MJ33 on manifestations of acute lung injury. Results showed that MJ33 could inhibit reactive oxygen species generation by lungs when measured LPS treatment. LPS at either a low or high dose significantly increased lung infiltration with inflammatory cells, secretion of proinflammatory cytokines, expression of lung vascular cell adhesion molecule, lung permeability, tissue lipid peroxidation, tissue protein oxidation, and activation of NF-κB. MJ33, given either concurrently or 2 h suquent to LPS, was able to significantly reduce all of these measured parameters
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
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文献和实验Abstract Previous studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in deficiencies in normal anterior-posterior development. To determine
at lower salt concentrations, whereas many primer pairs producing short amplification products worked better at higher salt concentrations. This is illustrated in the three figures below in which there is a realtive shift in the intensity of the products
DEVELOPMENTAL EFFECTS OF LITHIUM CHLORIDE ON XENOPUS EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos. INTRODUCTION: When the developing embryos of many organisms, such as the lytechinus variegatus (sea urchin
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