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- 保存条件：
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详见说明
- 库存：
99
- 供应商：
上海圻明生物
- 规格：
2μg
莫拉氏菌属通用PCR阳性对照质粒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验一家小公司的产品，不过实验室一直用，效果一直有保证，同期进行连接的其他同学也都连接成功。 结果： 转化后，平板上均有数量不等的菌落产生，少时4-5个，多时10来个。摇菌扩增8 h，进行菌液PCR检测（含阳性对照），检测组无阳性结果，但一直在1000 bp左右出现较弱条带，阳性组正常。提取质粒后，10 μl体系双酶切2 h，竟然在2000 bp和3000 bp处出现较弱条带（比Marker稍暗）。 特此悬赏10个叮当，请高手指点迷津。
直接检测 百日咳杆菌 PCR、巢式PCR 临床标本检测 结核分枝杆菌 PCR、SDA、Qbeta 肺结核的诊断、呼吸道标本检测、临床标本直接检测 鸟分枝杆菌复合群 PCR 鸟分枝杆菌和胞内分枝杆菌的区别 白喉棒状杆菌 PCR 产毒菌株的检测 肠致病性大肠埃希菌 多重PCR 产毒菌株检测 伤寒、副伤寒沙门菌 PCR 耐药质粒分型和检测 幽门螺杆菌 多重PCR 胃活检组织细菌检测 小肠结肠炎耶尔森菌 PCR 菌株分型 弯曲菌属 PCR 通过16s rRNA鉴定
直接检测 百日咳杆菌 PCR、巢式PCR 临床标本检测 结核分枝杆菌 PCR、SDA、Qbeta 肺结核的诊断、呼吸道标本检测、临床标本直接检测 鸟分枝杆菌复合群 PCR 鸟分枝杆菌和胞内分枝杆菌的区别 白喉棒状杆菌 PCR 产毒菌株的检测 肠致病性大肠埃希菌 多重PCR 产毒菌株检测 伤寒、副伤寒沙门菌 PCR 耐药质粒分型和检测 幽门螺杆菌 多重PCR 胃活检组织细菌检测 小肠结肠炎耶尔森菌 PCR 菌株分型 弯曲菌属 PCR 通过16s rRNA鉴定
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