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上海圻明生物
- 规格：
50次
Trichophyton rubrum红色毛癣菌PCR试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验红色毛癣菌 红色毛癣菌是毛癣菌属。红色毛癣菌是引致东南亚地区及澳洲北部的原住民体癣的真菌，且随着越战的发展，由返回国家的军人及难民被带至世界各地。现时世界各地大部份皮癣的病源多是由红色毛癣菌引致的医学教|育网搜集整理。局部性的皮癣，可用止痒杀菌的药物涂擦患处。
红色毛癣菌 红色毛癣菌是毛癣菌属下的一个种。红色毛癣菌是一种人源的皮癣菌，引起常见的皮肤浅部真菌病，如手癣、足癣、头癣等。 红色毛癣菌是毛癣菌属。红色毛癣菌是引致东南亚地区及澳洲北部的原住民体癣的真菌医学教育|网搜集整理，且随着越战的发展，由返回国家的军人及难民被带至世界各地。现时世界各地大部份皮癣的病源多是由红色毛癣菌引致的。局部性的皮癣，可用止痒杀菌的药物涂擦患处。
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