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上海圻明生物
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Dermacenter spp.革蜱PCR试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验体。我国主要媒介是全沟硬蜱，我国已证实有20省、市、自治区有本病流行。 ⑸Q热：病原体为贝氏立克次体（Coxiella burneti），牛、羊为人体Q热的主要传染源。感染方式主要由呼吸道吸入传播，也可通过消化道及蜱的叮咬、粪便污染伤口而感染。 ⑹北亚蜱传立克次体病：又称西伯利亚蜱传斑疹伤寒，草原革蜱为其主要媒介，我国新疆、内蒙、黑龙江有本病存在。 ⑺细菌性疾病：蜱能传播一些细菌性疾病，如鼠疫、布氏杆菌病、野兔热。
体。我国主要媒介是全沟硬蜱，我国已证实有20省、市、自治区有本病流行。 ⑸Q热：病原体为贝氏立克次体（Coxiella burneti），牛、羊为人体Q热的主要传染源。感染方式主要由呼吸道吸入传播，也可通过消化道及蜱的叮咬、粪便污染伤口而感染。 ⑹北亚蜱传立克次体病：又称西伯利亚蜱传斑疹伤寒，草原革蜱为其主要媒介，我国新疆、内蒙、黑龙江有本病存在。 ⑺细菌性疾病：蜱能传播一些细菌性疾病，如鼠疫、布氏杆菌病、野兔热。
为四种类型：①单宿主蜱：发育各期都在一个宿主体上，雌虫饱血后落地产卵。如微小牛蜱（Boophilus microplus）。②二宿主蜱：幼虫发育为若虫在一个宿主体上，而成虫在另一个宿主体上寄生。如残缘璃眼蜱（Hyaloma detritum）。③三宿主蜱：幼虫、若虫、成虫分别在3个宿主体上寄生。如全沟硬蜱、草原革蜱。90％以上的硬蜱为三宿主蜱，蜱媒疾病的重要媒介大多是三宿主蜱。④多宿主蜱：幼虫、各龄若虫和成虫以及雌蜱每次产卵前都需寻找宿主寄生吸血，每次饱血后离去。通常软蜱都属多宿主蜱
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