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Medulla junci灯心草PCR鉴定试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验延髓 medulla oblongata 亦称延脑。是脊椎动物菱脑的后半部即脑的最下部，与脊髓最上方相连的部分。因变得比脊髓膨大，所以又称脑球（ bulbus cerebri），其外形与脊髓很相似。腹侧面有橄榄体（ oliva），锥体交叉〔（ decu－ ssatio pyramidum），背侧有联络小脑的索状体（ corpus restiforme，也称为延髓小脑脚（ crusmedullocerebellare）、下小脑脚（ pedunculuscerebellaris
形成肾上腺中心部的组织。在宽阔的血管间隙中排列着形状不规则的细胞，其中也含有网状内皮系统的一部分。在交感神经的支配下，能分泌肾上腺素。在此意义上，肾上腺髓质是将神经信息转换为激素信息的一种神经内分泌转换器（neuroendocrine tra－nsducer）。肾上腺髓质最重要的作用，认为是在紧急情况时，通过交感神经为机体创造逃走或准备斗争的体内条件。此称为坎农（W.B.Cannon，1928）的应急学说。
位于肾上腺中央的内分泌腺，与皮质网状带的分界参差不齐。腺细胞内含有易被重铬酸钾氧化成棕黄色的颗粒，称嗜铬细胞。嗜铬细胞排列成团或索状，互相连接成网，网眼中有血窦和结缔组织。血窦互相汇合，至髓质中央形成中央静脉。嗜铬细胞可分为肾上腺素细胞和去甲肾上腺素细胞；前者数量多，排列成团，分泌肾上腺素；后者数量少，散在分布，分泌去甲肾上腺素。还有少量交感神经节细胞。肾上腺髓质对于维持生命并非必需，切除后，动物能生存较长时间，但丧失应急能力。
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