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- 欣润生物
- 江苏无锡
- IP4005-1
- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized pig colon epithelial cell l
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
巴马猪
- 组织来源：
结肠组织
- 相关疾病：
无
- 物种来源：
猪
- 免疫类型：
不详
- 细胞形态：
上皮型
- 是否是肿瘤细胞：
否
- 器官来源：
结肠
- 运输方式：
常温
- 年限：
新生
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化猪结肠上皮细胞简介：
产品描述：结肠分升结肠、横结肠、降结肠和乙状结肠4部分，大部分固定于腹后壁，结肠的排列酷似英文字母“M”，将结肠包围在内。结肠横切面由内到外依次为：黏膜（上皮层、固有层、黏膜肌层），黏膜下层（疏松结缔组织），肌层（内环形、外纵行两层平滑肌），外膜（纤维膜或浆膜）。肠黏膜上皮细胞是机体内外环境的重要屏障，持续暴露于大量抗原中，也是机体面对病原微生物的第一道防线。因此，肠黏膜上皮细胞除有吸收、分泌和转运等重要生理功能之外，在黏膜先天性和获得性免疫防御机制中也起着重要作用。肠黏膜上皮细胞作为首先接触抗原的细胞，在黏膜免疫反应的起始阶段发挥关键作用，它决定黏膜免疫反应的发生、性质和强度。探讨正常结肠黏膜上皮细胞的分离、体外培养方法，为研究肠黏膜上皮细胞以及与肠黏膜相关疾病建立合适的细胞模型。
产品货号：IP4005
产品类型: 永生化细胞
传代能力: 30代左右
产品形态:上皮型
培养基：永生化猪结肠上皮细胞完全培养基
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周左右货期
CK-18抗体免疫荧光染色鉴定
Establishment of conditionally immortalized epithelial cell lines from both colon and small intestine of adult H-2Kb-tsA58 transgenic mice
Intestinal mucosal cells have proved difficult to culture in vitro. Many attempts have been made to develop long-term cultures of these cells either by direct culturing or by attempting to immortalize these cells by using a range of transforming viral genes, but with little success. The recent development of a transgenic mouse bearing a temperature-sensitive mutation of the simian virus 40 large tumor antigen gene (tsA58) has enabled us to initiate conditionally immortalized cultures of epithelial cells from both small intestinal and colonic mucosa of adult mice. Crypts were isolated from either the small intestines or colons of young adult mice and cultured at the permissive temperature (33C) in medium containing conditioned medium from a human colon carcinoma cell line, LIM1863. Crypts from both tissues yielded cultures of epithelial cells that have now been in culture for more than 12 months with regular passaging. The epithelial nature of the cells has been confirmed by staining with anti-keratin antibodies. The intestinal origin of the cells was demonstrated by the ability of the cells to synthesize l
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
Immunity：「填补教科书」董晨院士团队解析 IL-17D 重要功能，并首次发现其受体
分泌的呢？作者通过分离小鼠结肠组织中的 CD45 + 和 CD45 - 细胞来探索 IL-17D 细胞来源，结果发现 CD45 - 细胞（以结肠上皮细胞为主）高表达 Il17d mRNA，提示结肠上皮细胞可能是 IL-17D 的主要来源。这一结果也通过免疫荧光染色和骨髓嵌合体小鼠模型得到了进一步的证实。 图片来源：Immunity「我要到哪里去」IL-17D 作用于什么细胞而发挥功能？进一步地，作者研究了 IL-17D 作用的靶细胞。IL-22 及其下游效应蛋白，如 RegIIIb 和 RegIIIg
比如，所谓的在肿瘤里面有表达，你用什么方法测定的？是否真的表达了？是不是由于发达的肿瘤微血管，转移来了“客人”，被你巧遇上了？:D 或者，这个蛋白是一种分泌蛋白？全部的蛋白都分泌到了胞外，而细胞内含量低于检测限？ bigbang_0_0 有可能是旁分泌蛋白 茯苓猪爱 有可能是旁分泌产生的蛋白.研究的时候可以根据实验目的构建表达载体,解决细胞系不表达的问题. bigbang_0_0
Nature 重磅！中山大学郑灿镔等人的研究成果为异种移植提供新思路
，并没有观察到明显的细胞竞争的现象，也无明显的人细胞凋亡现象。这些结果表明，人类和小鼠启动细胞间的竞争可能不通过分泌因子，而是通过直接接触实现的。 图片来源：Nature上述结果表明，细胞凋亡是细胞竞争中消除「弱势」细胞的主要机制，那么阻断细胞凋亡是否能克服细胞竞争这种现象呢？为此，研究人员构建了稳定表达 BCL-2 的细胞系 BCL-2OE-hiPSCs。结果发现，在与 mEpiSCs 共培养过程中，BCL-2 过表达可以有效防止人源细胞的消除；同样的，敲低或敲除促凋亡基因 Tp53 亦能完全挽救
技术资料