- ￥600 - 3200
- 上海莼试
- CS-01Y72765
- 国产
- 2025年07月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
29
- 英文名：
Resazurin sodium salt
- CAS号：
62758-13-8
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
1g 5g
分子式:C12H6NNaO4
分子量:251.17
溶解度:≥ 25.1mg/mL in DMSO
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
The non-fluorescent resazurin has been used as a fluorogenic oxidation-reduction indicator in various cells by flow cytometry, fluorescence microscopy and high-throughput screening. Its red-fluorescent product, resorufin, has absorption/emission maxima ~575/585 nm.In vitro: Resazurin has been applied as a simple and versatile way to measure cell proliferation and cytotoxicity. Although resazurin presented plenty of advantages over other cytotoxicity or proliferation tests, everal drawbacks to the routine use of resazurin were also orved. Previoius studies showed accumulation of the fluorescent product of resazurin in the medium which could result in an overestimation of cell population. Moreover, the extensive reduction of resazurin by metabolically active cells would lead to a final nonfluorescent product, and thus an underestimation of cellular activity [1]. In order to evaluate the effect of resazurin over longer incubation times, MCF7, MCF10A, 3T3-L1 and D1 cell lines were tested. Results showed that MCF7, 3T3-L1, and D1 cells cultured for 8 days with 20% resazurin had significantly less cell survivability. Moreover, levels of metabolic activity, DNA concentration, as well as glucose consumption of the cell lines in medium with 20% resazurin were significantly lower. Data also showed that the toxicities were more pronounced in the cancer cell lines than in the noncancerous cells [2].
商品属性：
| 货号 | CS-01Y72765 | 规格 | 1g 5g |
| CAS号 | 62758-13-8 | 分子量 | 251.17 |
| 含量 | >98.00% | 别名 | |
| 分子式 | C12H6NNaO4 | 化学名 | sodium 7-oxido-3-oxo-3H-phenoxazine 10-oxide |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
| Recombnant Human PEBP1 proten | Recombnant Human FAM20A, N-H |
| Recombnant Human HMOX1 proten | 磷酸化马铃薯球蛋白(结节性硬化)封闭多肽 |
| Recombnant Human GOT1 proten ,C- H Tag | 周期素依赖性激酶1封闭多肽 |
| Recombnant Human POU2F1, N-H | 腺苷单磷酸活化蛋白激酶α1封闭多肽 |
| Recombnant Human NME6, N-H | 胞浆亮氨酸tRNA连接酶封闭多肽 |
| Recombnant Human CD20/M4A1, N-H | 蛋白赖氨酸-6-氧化酶封闭多肽 |
| Recombnant Human THB3 proten | 磷酸化细胞核因子NF-κB p65封闭多肽 |
| Recombnant Human FLNB, N-H | 磷酸化FANCD2封闭多肽 |
| Recombnant Moue CCL2/MCP-1 proten ,No Tag | 肝细胞粘附分子2封闭多肽 |
| Recombnant Human tratfn proten ,No Tag | 小肠型脂肪酸结合蛋白封闭多肽 |
| Recombnant Human BDNF, N-H | 整合素alpha E2 封闭多肽 |
| Recombnant Human FKBPL proten | C-K封闭多肽 |
| Recombnant Human FGF19 proten | Resazurin sodium salt内着丝粒蛋白封闭多肽 |
| Recombnant Human HLA-G proten | 生长激素诱导跨膜蛋白封闭多肽 |
| 抑癌基因5封闭多肽 | 肌动蛋白结合蛋白2B封闭多肽 |
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Epithelial Sodium Channel in Planar Lipid Bilayers
Amiloride-sensitive Na+ channels belong to the epithelial Na+ channel (ENaC)-degenerin superfamily of ion channels. In addition to their key role in sodium handling, they serve diverse functions in many tissues. Improper functioning of ENaC
Sodium Acetate Precipitation of Small Nucleic Acids
实验步骤 1. Add 2 μl carrier to the nucleic acid solution and mix well. 2. Add 1:10 volume of 3 M sodium acetate and mix thoroughly; for 230 μl of Lower Running Buffer, this will be 23 μl of 3 M
Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
技术资料暂无技术资料 索取技术资料
