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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human CD81 antigen (CD81), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human CD81 antigen (CD81), partial (Active)品名简称:
Recombinant Human CD81 protein, partial (Active)货号:
CSB-MP004960HUd7规格:
20μg价格:
816LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Measured by its binding ability in a functional ELISA. Immobilized Human CD81 at 2 μg/mL can bind Anti-CD81 recombinant antibody(CSB-RA004960MA1HU), the EC50 is 4.166-5.578 ng/mL.基因名:
CD81Uniprot No.:
P60033别名:
TAPA1;TSPAN28种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
11.1 kDa表达区域:
113-201aa氨基酸序列:
FVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGK蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells.功能2:
/文献:
"TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins." Oren R., Takahashi S., Doss C., Levy R., Levy S. Mol. Cell. Biol. 10:4007-4015 (1990)研究领域:
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文献和实验和其他膜蛋白进行了优化。用Rho1D4系统纯化后的膜蛋白通过凝胶过滤以除去用于洗脱的多肽。使用此纯化系统的科研人员均反馈蛋白回收量均达到毫克级。(见表1)3,4,5,6,7,8纯化的膜蛋白可用于功能性研究纯化的蛋白可用于功能性研究,如配体结合的特性,蛋白与蛋白间的相互作用。例如,将标记过的ABCA4固定在载有Rho1D4抗体的基质上来确定它与天然配体(一种视网膜的加成物)的亲和结合特性,加入ATP后即释放2。又例如,CD81蛋白被整体固定在涂有Rho1D4抗体的平板上,它表现出和分离可溶蛋白片段与丙型
体? 外泌体鉴定是进一步研究外泌体功能和机制的工作基础。目前的鉴定方法主要包括透射电子显微镜(TEM)进行形态学观察、纳米颗粒追踪分析(NTA)进行粒径和密度分析、用 WB 进行蛋白标志物的鉴定。WB 鉴定包括 CD63、CD9、CD81、TSG101、Hsp70、syntenin1 等。 透射电镜图 纳米颗粒追踪分析 WB 五、如何研究外泌体? 外泌体的研究方向主要包括以下三个方面: 寻找疾病诊断和预后的分子标志物。 揭示疾病发病机制,从微环境角度解释
研究的集大成者。2006年,洛克菲勒大学病毒和传染病实验室主任查尔斯·莱斯和同事首次成功地在实验室制造出了一种能让动物感染上的丙肝病毒。最近,莱斯、普洛斯和同事发现,丙肝病毒感染需要以前科学家找出的丙肝病毒入口因子CD81和B族I型清道夫受体这两个蛋白以及两个紧密连接蛋白claudin 1和occludin的帮助。在此前的研究中,洛克菲勒大学的科学家就已经证明,人体CD81和occludin这两种蛋白在丙肝病毒进入老鼠细胞的过程中起着重要作用。 在新研究中,洛克菲勒大学和斯克里普斯研究所的科学
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