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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Paraneoplastic antigen Ma2 (PNMA2), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥19668.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥8868.0 |
| 规格: | 20μg | 产品价格: | ¥3168.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
PNMA2Uniprot No.:
Q9UL42别名:
40 kDa neuronal protein;Onconeuronal antigen Ma2;Paraneoplastic neuronal antigen MM2种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Baculovirus分子量:
43.9 kDa表达区域:
2-361aa氨基酸序列:
ALALLEDWCRIMSVDEQKSLMVTGIPADFEEAEIQEVLQETLKSLGRYRLLGKIFRKQENANAVLLELLEDTDVSAIPSEVQGKGGVWKVIFKTPNQDTEFLERLNLFLEKEGQTVSGMFRALGQEGVSPATVPCISPELLAHLLGQAMAHAPQPLLPMRYRKLRVFSGSAVPAPEEESFEVWLEQATEIVKEWPVTEAEKKRWLAESLRGPALDLMHIVQADNPSISVEECLEAFKQVFGSLESRRTAQVRYLKTYQEEGEKVSAYVLRLETLLRRAVEKRAIPRRIADQVRLEQVMAGATLNQMLWCRLRELKDQGPPPSFLELMKVIREEEEEEASFENESIEEPEERDGYGRWNHE蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Tags & Cell MarkersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
family (4 ), however, in vitro DNA-PK acts as a serine/threonine protein kinase (5 –3 ). The physiological substrates of DNA-PK are not known, however in vitro DNA-PK is known to phosphorylate many proteins including hsp90, SV40 large T antigen, serum
4) 配制Tris缓冲液 由一定量的Tris HCl(Tris酸)和Tris Base(Tris碱)混合而得。 5) 重量与摩尔数转换工具 已知一蛋白的分子量(kDa),可由重量推算出摩尔数和分子数,以及在一定体积内摩尔浓度。相反,也可由一个蛋白的摩尔数推算出重量和分子数,以及由摩尔浓度推算出所含的蛋白质量。 附:这些缓冲液在溶解PeproTech公司重组细胞因子和蛋白中的应用举例 1) 磷酸盐缓冲液 1 x PBS用于溶解100
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
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