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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Antithrombin-III (SERPINC1) (R294C)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Antithrombin-III (SERPINC1) (R294C)品名简称:
Recombinant Human SERPINC1 protein (R294C)货号:
CSB-EP021079HU(M1)规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
SERPINC1Uniprot No.:
P01008别名:
Serpin C1种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
58.5 kDa表达区域:
1-464aa(R294C)氨基酸序列:
MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRCVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.功能2:
/文献:
"Antithrombin III Utah: proline-407 to leucine mutation in a highly conserved region near the inhibitor reactive site." Bock S.C., Marrinan J.A., Radziejewska E. Biochemistry 27:6171-6178(1988)研究领域:
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
. Sequence Data Analysis Guidebook Edited by Swindell, Simon R., 1996 71. PRINS and In Situ PCR Protocols Edited by Gosden, John R., 1996 72. Neurotransmitter Methods Edited by Rayne, Richard C., 1997 73. Neuropeptide Protocols Edited by Irvine, G
基(III) hCOs 形成培养基(I) hCOs 形成培养基(II) hCOs 形成培养基(III) hCOs 形成培养基(IV) 近岸蛋白产品货号 基础培养基 DMEM-F12 RPMI-1640 RPMI-1640 RPMI-1640 RPMI-1640 RPMI-1640 DMEM-F12 RPMI-1640 RPMI-1640 RPMI-1640 penicillin-streptomycin
选用NA(N21)或NAR(N17)YNN(R表示嘌呤,Y表示嘧啶),但在合成时,siRNA的正义链3’端需用dT—dT代替。TuscRl等建议设计的siRNA不要针对mRNA的5’和3’端非编码区,因为这些区域有丰富的调控蛋白结合位点,而UTR结合蛋白或者翻译起始复合物可能会影响siRNP(siRNA protein complex,核酸内切酶复合物)结合mRNA,从而影响siRNA干扰的效果。最后还应将候选siRNA序列在GenBank进行BLAST检索,与非同源基因具有3个或3个以上碱基相异
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