Catenin beta Phospho-Tyr142 ra

【求助】关于gsk3-beta功能的疑问

的关系理不出头绪。GSK-3 beta 总蛋白减少，p-GSK-3 beta (ser 9)增加，是不是足以说明有活性的GSK-3 beta 减少更多，这样理解不知道对不对，希望给以指点。 galaxy22 楼主你好，请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的，active B catenin是减少的，那我有必要做ser9 和tyr216的磷酸化形式吗？我想研究wnt和akt。。。 棉花糖的温柔

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known

v468 chapter 5 糖原合成酶激酶-3 的抑制

at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase