P4R3B rabbit pAb

v468 chapter 5 糖原合成酶激酶-3 的抑制

), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG

Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

; * p Fig. 4.3. IGF1, but not Wnt3a, reduces cellular GSK3b activity. SH-SY5Y cells werestimulated with IGF1 or Wnt3a (1 h), then harvested in cell lysis buffer. GSK3b wasimmunoprecipitated from lysates and its activity was measured in an in vitro