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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
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- 英文名:
N-(1-Oxoheptyl)glycine Ethyl Ester
- 库存:
1
- 供应商:
上海甄准
- CAS号:
574010-47-2
- 规格:
500mg
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文献和实验Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
100 mM N ‐hydroxysuccinimide (NHS) 400 mM N ‐ethyl‐N ‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride (EDC)
I. 0.25 N HCl: 21.5 ml conc. HCl (11.6N) to 1 liter volume. II. 0.5 M NaOH, 1.5 M NaCl: 87.7 g NaCl, 20.0 g NaOH to 1 liter volume. III. 1.0 M Tris, 1.5 M NaCl: 121.1 g Tris base, 87.7 g NaCl, pH to 8.0 with conc. HCl (~50 ml), adjust to 1 liter
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
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