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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Colorimetric) (96 assays)
- 规格:
96次分析
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文献和实验Materials Cells of interest [Methyl 3 H]thymidine (20 Ci/mmol) or [2‐14 C]thymidine (50 mCi/mmol
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
histone deacetylation, resulting in increased global acetylation levels. Once the ChIP protocol has been established, it has to be determined which histone modifications will be analyzed. For this purpose, data obtained from immunocytochemistry
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