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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100 µl
- 保存条件：
-20 C
- 供应商：
西宝生物
- 英文名：
CELL LYSATE RAT BRAIN
- 规格：
IMMUNOBIOLOGICALS
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文献和实验the supernatant to a fresh centrifuge tube and discard the pellet. 5.Dilute the cell lysate at least 1 : 10 before determining the protein concentration because of the interference of the detergents in the lysis buffer with the Coomassie-based reagent
Purification of Endothelial Cells from Rat Brain
of the cerebrocortical endothelial cell population is highly desirable. For this purpose, rat brains are mechanically minced and subsequently digested enzymatically with collagenase. In this protocol, the capillary fraction (microvessels) and the fraction enriched
In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
The identification of specific messenger RNA molecules and the characterization of the proteins encoded by them has been greatly assisted by the development of in vitro translation systems. These cell-free extracts comprise the cellular
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