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- 英文名:
2-Thiazolecarboxaldehyde
- 库存:
1
- 供应商:
上海甄准
- CAS号:
10200-59-6
- 规格:
1g
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文献和实验DNA 溶液的Eppendorf 管置于冰上。 (2)PCR扩增反应 (a)使用QIAGEN 的PCR Core Kit ,用四种不同的引物对来扩增纯化的DNA 。管A 含叶绿体DNA 特异性引物,管B 含LeCtin特异性引物,管C含NOS终止子特异性引物,管D含35S启动子特异性引物。每一管依次加入下列溶液: 33.9μl 的MilliQ水(总体积达50μl ),5μl10×PCR 缓冲液(100mM Tris-HCl buffer ,pH 8;500mM KCl ;15mM MgCl
. 1 ). This chapter describes the measurement of NOS-functional activity by determining the NOS inhibitor-sensitive conversion of radiolabeled (14 C or 3 H) arginine to citrulline, with separation of the labeled product from substrate by ion-exchange techniques
A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I
In addition to its well-known cofactor roles for aromatic amino-acid hydroxylases, an essential role of tetrahydrobiopterin (BH 4 ) for nitric oxide synthases (NOS) has been recently established ( 1 – 3 ). All three isoforms of NOS contain
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