MOUSE INTERFERON-gamma (M-IFN-

The major interferon is produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of classII histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens or alloantigens. It isproduced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic and EGF. Recombinant Murine IFN-gamma produced in E.coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15,601 Dalton.