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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) (96 assays)
- 供应商:
上海西宝
- 规格:
96次分析
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文献和实验cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem
by the addition of a detergent results in the liberation of the crystals which are solubilized. The color can then be quantified using a simple colorimetric assay. The results can be read on a multiwell scanning spectrophotometer (ELISA reader). The MTT Cell
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates and Extracts
to provide proof of ACTase activity; radioactive tracer analysis was the most sensitive technique and a microtitre-based colorimetric assay was the most cost-and time-efficient for large scale analyses. Freeze-thawing was adopted as the preferred method
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