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- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
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咨询甄准
- 英文名:
25-O-Deacetyl-23-O-acetyl Rifabutin
- 库存:
1
- 供应商:
上海甄准
- CAS号:
1242076-43-2
- 规格:
25mg
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文献和实验Replication timing by comparative hybridization
of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 6. Hold at 37o C for 90-120 min (until budded cells accumulate). Remove one sample (t = 0), then swirl the flask in ice-water and return it to 23o C. Continue
200ml PEG6000 40% 37.21 4.0g 20g 40g 60g 80g H2 O mQ 62.79 6.75g 33.75g 67.5g 101.25g 135.0g p=1.075. PMSF (store at -20o C) Conc. Stock 20ml C7 H7 FO2 S 100mM 174.2g/M
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
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