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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
EpiQuik DNA Methyltransferase 1 Activity/Inhibitor Screening Assay Core Kit (48 assays)
- 供应商:
上海西宝
- 规格:
48次分析
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文献和实验has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity in small,snap frozen pieces of tissue (e.g.,colon polyps),or in cultured cells. 1.Homogenize Cells/Tissues For cells in culture: Harvest
Quantitate proteins in 15 ml aliquots of lysates (in duplicate) by using a Bradford assay (I have been using the Biorad kit), using BSA as standard. Don't forget to add 15 ml LB to each BSA sample and to the blank tube. Use the same LB batch as that used
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
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