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- 2025年07月12日
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- 技术资料
- 保存条件：
咨询甄准
- 保质期：
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- 英文名：
4-Bromo-5-chloro-2-methoxyaniline
- 库存：
1
- 供应商：
上海甄准
- CAS号：
102170-53-6
- 规格：
500mg
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文献和实验Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
colonies. If possible as a positive control use 1-5 ng of a plasmid (e. g. pBR322) that previously has transformed this batch of competent cells efficiently. 3.Place competent cells directly on ice after removing from -80 degrees C storage. As the cells
Methods for use with the mTn-lacZ/LEU2-mutagenized library
empirically. Filters are placed colony-side up onto X-Gal plates (120 ug/ml 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 0.1 M NaPO4 [pH 7] and 1 mM MgSO4 in 1.6% agar) and incubated at 30o C for up to 2 days. These plates
Methods for use with the mTn-3xHA/lacZ-mutagenized library
-side up onto X-Gal plates (120 ug/ml 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 0.1 M NaPO4 [pH 7] and 1 mM MgSO4 in 1.6% agar) and incubated at 30o C for up to 2 days. These plates can be very thin; their use increases the signal over that obtained
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