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- 英文名：
4-Hydroxybutanesulfonate Sodium Salt (Technical Grade)
- 库存：
1
- 供应商：
上海甄准
- CAS号：
31465-25-5
- 规格：
500mg
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
endonuclease buffer 3 M sodium acetate ( appendix 22 ) 95% and 70% ethanol, –20°C
in a high salt buffer and heated briefly to 65-70 °C to disrupt secondary structures. Afterwards, annealing to oligo-dT is performed at room temperature. The oligo-dT molecules are linked to a carrier which allows washing off non-bound nucleic acids
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
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