Mouse Anti Human IgG Fab - AF5

Measurement of HAMA and Anti-Idiotypic Antibodies

). Even in the absence of such immunization some patients may possess HAMA activity because of polyclonal IgM rheumatoid factors that crossreact with Fc epitopes on both human and mouse IgG (1 ).

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum

Intraspinal Transplantation of Mouse and Human Neural Precursor Cells

the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing