Rabbit Anti Human IgM - AF555

Anti-GFP/BrdU In Situ Immunostaining

Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative:  4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit

Measurement of HAMA and Anti-Idiotypic Antibodies

). Even in the absence of such immunization some patients may possess HAMA activity because of polyclonal IgM rheumatoid factors that crossreact with Fc epitopes on both human and mouse IgG (1 ).

Production of a Human Antibody Library in the Phage-Display Vector pSEX81

Human monoclonal antibodies (MAbs) are more suitable than MAbs of animal origin for clinical applications because of lower hypersensitivity reactions, less formation of circulating immune complexes and lower anti-immunoglobulin responses