AurB/C (Phospho-Thr236/202) 多克

运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白

using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative

【求助】关于gsk3-beta功能的疑问

a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search

In vitro transcription with yeast nuclear extract

buffer 500 mM Potassium Acetate, pH 7.6100 mM HEPES, pH 7.6 5 mM EDTA 25 mM MgOAc Creatine phospho kinase (sigma C-3755 165 units/mg) 2.0 mg/ml in HA + 0.1 M potassium acetate Stored in 12 microliter aliquots at -70 degrees. The enzyme is stable