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- 2025年07月13日
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- 技术资料
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100
- 供应商：
艾美捷科技
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Total CYP26A1 Cell-Based ELISA试剂盒，Total CYP26A1 Cell-Based ELISA Kit，Total CYP26A1 Cell-Based ELISA Kit,Total CYP26A1 Cell-Based ELISA Kit
产品名称：Total CYP26A1 Cell-Based ELISA试剂盒-Total CYP26A1 Cell-Based ELISA Kit
产品货号：BGT-KET-12491
产品规格：96wells
Total CYP26A1 Cell-Based ELISA Kit,Total CYP26A1 Cell-Based ELISA Kit
Total CYP26A1 Cell-Based ELISA Kit查看提供的产品datasheet。
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
, and protein content into circulation because of turnover and breakdown of cell structures. Proteins in solution most likely circulate as complexes, which protects them from degradation. We describe a cell-free, bead-based method that takes advantage
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