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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
艾美捷科技
- 检测范围:
查看datasheet
- 应用:
elisa
- 样本:
查看datasheet
Total SENP1 Cell-Based ELISA试剂盒,Total SENP1 Cell-Based ELISA Kit,Total SENP1 Cell-Based ELISA Kit,Total SENP1 Cell-Based ELISA Kit
产品名称:Total SENP1 Cell-Based ELISA试剂盒-Total SENP1 Cell-Based ELISA Kit
产品货号:BGT-KET-12021
产品规格:96wells

Total SENP1 Cell-Based ELISA Kit,Total SENP1 Cell-Based ELISA Kit

Total SENP1 Cell-Based ELISA Kit查看提供的产品datasheet。

Total SENP1 Cell-Based ELISA Kit产品的保存方式查看提供的产品datasheet。
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内,每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激,我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤,分别固定,打孔,猝灭,封闭,然后一抗孵育,二抗孵育,最后显色,用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正,最后得出以上数据。Quantitative
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
, and protein content into circulation because of turnover and breakdown of cell structures. Proteins in solution most likely circulate as complexes, which protects them from degradation. We describe a cell-free, bead-based method that takes advantage
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