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- Miltenyi Biotec
- 130-091-052
- 2025年10月03日
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臻诺生物
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QuadroMACS 15 ml Tube Rack
美天旎 130-091-052 QuadroMACS 15 ml Tube Rack 现货供应
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文献和实验Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
Shake until the solutes have dissolved. Adjust the pH to 7.0 with 5 N NaOH (~0.2 ml). Adjust the volume of the solution to 1 liter with deionized H2 O . Sterilize by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2 ) on liquid cycle. SOB
Freezing Worms by Michael Koelle 4/6/94 I. The preferred method 1. Wash worms off of 1 large plate or 3 small plates in 3 mls S Basal into a sterile 15 ml disposable centrifuge tube. Worms should be harvested
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