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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Transcriptional repressor CTCFL protein (1-270AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q8NI51
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, IHC; Recommended dilution: IHC:1:20-1:200
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
CTCFL
- 抗体英文名:
CTCFL Antibody
- 抗体名:
Zinc finger protein CTCF-T antibody
- 规格:
100μl/50μl/20μl
| 规格: | 100μl | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥880.0 |
| 规格: | 20μl | 产品价格: | ¥440.0 |
保存缓冲液
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.功能
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 \'Arg-3\' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验-mercaptoethanol to 0.1%, glusulase to 0.05%, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37o C. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts
由淋巴细胞杂交瘤产生的、只针对复合抗原分子上某一单个抗原决定簇的特异性抗体。淋巴细胞杂交瘤是用人工方法使骨髓瘤细胞(纯系小鼠的腹水瘤型浆细胞)与已用抗原致敏并能分泌某种抗体的淋巴细胞(常用致敏动物的脾细胞,起作用的是其中的B细胞)融合而成的。用来使上述淋巴细胞致敏的抗原有人和动物的T细胞、B细胞、红细胞、肿瘤细胞、各种微生物或其他抗原物质等。这种融合细胞既具有肿瘤细胞能大量、无限繁殖的特性,又具有B细胞能合成分泌特异性抗体的能力。由于一个B细胞克隆只针对一个抗原决定簇产生
淋巴系细胞有B细胞和 T细胞两个系列,其中 B细胞是抗体形成细胞前体( antibody- forming cellprecursor),在胞膜表面具有以单一特异性抗体作为抗原受体,一旦与抗原结合就直接或借助于 T细胞进行增殖分化,结果成为高速度合成和分泌具有与受体同样特异性抗体的细胞,这些细胞总称为抗体形成细胞。 IgG抗体(免疫球蛋白 G), IgA抗体, IgE抗体(或许 IgD抗体)是在浆细胞内形成的。形成 IgG的浆细胞在脾及淋巴结等末梢淋巴系组织和肠粘膜固有膜和肺间质等全身
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