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- 供应商:
路明克斯
Luminex Corporation成立于1995 年,总部位于美国德克萨斯州的奥斯汀,是一家专注于研发和生产高科技生物检测分析仪器及流式细胞仪并将其运用于生命科学研究及临床诊断领域的公司。公司致力于提供创新性和突破性的解决方案,帮助客户改善诊疗水平和促进全球范围内的科学进步。服务范围包括临床诊断、药物开发、基因组学和蛋白质组学研究、个性化医学、生物防卫研究以及食品安全等等,以满足不同领域、多样化市场的客户需求。
主营产品:Amnis® FlowSight®成像流式细胞仪、Guava® easyCyte™微毛细管流式细胞仪、Guava® Muse®细胞分析仪等产品。
路明克斯(Luminex)常用试剂,试剂盒
| Kits & Reagents (Guava) | ||||
| 4000-0040 | Guava ViaCount Reagent (40 mL) | |||
| 4000-0041 | Guava ViaCount Reagent (240 mL) | |||
| 4000-0061 | Guava Express 7-AAD Reagent | |||
| 4200-0140 | Guava Instrument Cleaning Fluid (ICF) | |||
| 4500-0020 | Guava Check Kit | |||
| 4500-0025 | Guava easyCheck Kit | |||
| 4500-0110 | Guava ViaCount Flex | |||
| 4500-0220 | Guava Cell Cycle Reagent | |||
| 4500-0450 | Guava Nexin Reagent (100 tests) | |||
| 4500-0455 | Guava Nexin Reagent (500 tests) | |||
| 4700-0050 | Guava ViaCount Cell Dispersal Reagent | |||
| 4700-0060 | Guava ViaCount Flex Reagent | |||
| CN-0444-01 | Flow Cell, Guava easyCyte SL systems (ECSL0500-2270) | |||
| CN-0448-01 | Flow Cell, Guava easyCyte HT Systems (ECHT0500-2260) | |||
| FCCH100106 | Guava MitoDamage Kit | |||
| FCCH100108 | Guava Annexin Red Kit | |||
| FCCH100171 | Guava Autophagy LC3 Antibody-based Assay Kit | |||
| FCCS025153 | Guava H2A.X DNA Damage Dual Detect Kit | |||
| FCCS100182 | Guava H2A.X Phosphorylation Kit | |||
| FCPA100101 | Guava SARS-CoV-2 Multi-Antigen Antibody Assay | |||
| Accessories/Other (Muse) | ||||
| 6000-2890 | Travel Case, Muse | |||
| CN-0452-01 | Muse Cleaning Solution Bottle (M0110-7245) | |||
| CN-0453-01 | Muse Waste Solution Bottle (M0110-7250) | |||
| CN-0454-01 | Muse Flow Cell Assembly, (M0500-3120) | |||
| CN-0455-01 | Muse Flow Cell Cleaning Tool (M6000-2820) | |||
| CN-0457-01 | Tubes, Eppendorf, 1.5ml Bag of 500 | |||
| FC-0390-01 | Muse Yearly PM Kit, 0110-8569 | |||
| FC-0396-01 | 1.5 mL Eppendorf tube O-ring caps, 500 per pkg (M1000-3675) | |||
| Kits & Reagents (Muse) | ||||
| MCH100101 | Muse System Check Kit | |||
| MCH100102 | Muse Count & Viability Kit (40 mL) | |||
| MCH100104 | Muse Count & Viability Reagent (200x) | |||
| MCH100105 | Muse Annexin V & Dead Cell Kit | |||
| MCH100106 | Muse Cell Cycle Kit | |||
| MCH100107 | Muse Cell Dispersal Reagent | |||
| MCH100108 | Muse Caspase-3/7 Kit | |||
| MCH100109 | Muse MultiCaspase Kit | |||
| MCH100110 | Muse MitoPotential Kit | |||
| MCH100111 | Muse Oxidative Stress Kit | |||
| MCH100112 | Muse Nitric Oxide Kit | |||
| MCH100114 | Muse Ki67 Proliferation Kit | |||
| MCH200101 | Muse H2A.X Activation Dual Detection Kit | |||
| MCH200102 | Muse EGFR-RTK Activation Dual Kit | |||
| MCH200103 | Muse PI3K Activation Dual Detection Kit | |||
| MCH200104 | Muse MAPK Activation Dual Detection Kit | |||
| MCH200105 | Muse Bcl-2 Activation Dual Detection Kit | |||
| MCH200107 | Muse Multi Color DNA Damage Kit | |||
| MCH200108 | Muse PI3/MAPK dual Activation Kit | |||
| MCH200109 | Muse Autophagy LC3-antibody based Kit | |||
| MCH600103 | Muse Count & Viability Reagent (240 mL) | |||
| MIM100101 | Muse Human CD4 T Cell Kit | |||
| MIM100102 | Muse Human CD8 T Cell Kit | |||
| MIM100103 | Muse Human B Cell Kit | |||
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文献和实验Affinity Column Preparation 亲和层析柱制备
concentrated HCl.14.Place 0.3 ml of 1M K2HPO4 into 15 eppendorf tubes.This will be used to immediately neutralize the antibody as it is eluted from the column.The antibody will denature at pH 2.8 over time,so it is important to neutralize as soon as possible.15
equal volume of phenol/chloroform then vortex. 5) Goto step 6 6) Spin for 10 min at 2,500 rpm in a bench centrifuge. 7) Transfer 0.7 ml of the aqueous phase to an Eppendorf tube, add 0.7 ml phenol/chloroform, vortex and spin for 5 min. 8) Transfer
into a 4" x 6" 500 guage (thick!) plastic bag. 2) Add 2-3 ml homogenization buffer (you may need more for large leaves) to the bag. 3) Grind the leaf inside the bag using the top end of a 50 ml corex tube. 4) Pour the homogenate immediately into a 15
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