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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Streptococcus agalactiae Chaperonin GroEL (groEL)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥12372.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2880.0 |
| 规格: | 20μg | 产品价格: | ¥1536.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
groELUniprot No.:
Q9AME7别名:
60 kDa chaperonin;Chaperonin-60;Cpn60种属:
Streptococcus agalactiae蛋白长度:
Full Length来源:
E.coli分子量:
64.2 kDa表达区域:
1-540aa氨基酸序列:
MAKDIKFSADARSAMVRGVDILADTVFVTLGPKGRNVVLEKAFGSPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLPQAIVREGLKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYISEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYILITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGAGDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKLRIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQIAYNAGYKGSVIIARLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVASLILTTEAVVANKPEPEAPTAPAMDPSMMGGF蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验)的折叠需要前肽( propeptide )的帮助。这类前肽常位于信号肽与成熟多肽之间,在蛋白质合成过程中与其介导的蛋白质多肽链是一前一后合成出来的,并以共价键相连接,是成熟多肽正确折叠所必需的,成熟多肽完成折叠后即通过水解作用与前肽脱离。 Shinde 和 Inouye 将这类前肽称为分子内伴侣( intramolecular chaperones )。分子伴侣主要分为: 伴侣素家族( chaperonin
作用,或者说它们给处于折叠中间态的多肽链提供了更多的正确折叠的机会,因而它们能提高折叠反应的产率而不一定能提高其速率。细胞内的蛋白质折叠与组装可以发生在胞浆、线粒体或内质网( endoplasmic retinum , ER ),因而分子伴侣可以存在于胞内的各个部位。分子伴侣主要分为以下几类: 1) 伴侣素家族(chaperonin, Cpn) Cpn 家族是具有独特的双层 7-9 元环状结构的寡聚蛋白( Hemminngwen 1988; Cheng 1989 ),它们以依赖 ATP
Strategies to Optimize Protein Expression in E. coli
as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL. Biochem. Biophys. Res. Commun. 333:686‐693. Dvir, H. and Choe, S. 2009. Bacterial expression
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