env/env蛋白Recombinant Simian re

纯度：

Greater than 95% as determined by SDS-PAGE.

基因名：

env

Uniprot No.：

P04027

别名：

Env polyprotein;SU;Glycoprotein 70;gp70;TM;Glycoprotein 20;gp20

种属：

Simian retrovirus SRV-1

蛋白长度：

Partial

来源：

Yeast

分子量：

42.0 kDa

表达区域：

23-395aa

氨基酸序列：

GFGDPREALLEIQQKHGKPCDCAGGYVSSPPTNSLTTVSCSTYTAYSVTNSLKWQCVSTPTTASPTHIGSCPSQCNSQSYDSVHATCYNHYQQCTIGNKTYLTATMIRDKSPSSGDGNVPTILGNNQNLIIAGCPENKKGQVVCWNSQPSVHMSDGGGPQDKVREIIVNKKFEELHKSLFPELSYHPLALPEARGKEKIDAHTFDLLATVHSLLNVSSQRQLAEDCWLCLRSGDPVPLALPYDNTSCSNSTFFFNCSNCSCLITPPFLVQPFNFTHSVCLYADYQNNSFDIDVGLAGFTNCSSYINISKPSSPLCAPNSSVFVCGNNKAYTYLPTNWTGSCVLATLLPDIDIIPGSEPVPIPAIDHFLGRPKR

蛋白标签：

C-terminal 6xHis-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane. ; The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.

内毒素：

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SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

In Stock Protein

Biological_Activity:

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Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

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Function:

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