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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Saccharomyces cerevisiae DNA repair protein RAD59 (RAD59)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Saccharomyces cerevisiae DNA repair protein RAD59 (RAD59)品名简称:
Recombinant Saccharomyces cerevisiae RAD59 protein货号:
CSB-EP619572SVG规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
RAD59Uniprot No.:
Q12223别名:
/种属:
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)蛋白长度:
Full Length来源:
E.coli分子量:
33.5 kDa表达区域:
1-238aa氨基酸序列:
MTIQAKPSSSISYDSTTYGTAPGLDIKEFQIIEDWNGRPASAWSVQRIGLLQSKIERYTYNIYHNNKYGKHNLSKLIPGHALIQFANETFGYDGWRMDVIDVEARECQPFTAVNNGENTNTSEVKYTVVAEAQVKVTLKDGTNTQCGGLGRITLSSRGECYNRSKKEAVGDALKKALLSFEKIILDYETKITNNYYVDGLYGSKKIKNEANTNYNLLSATNSKPTFIKLEDAKGTHIK蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing. Anneals complementary single-stranded DNA.功能2:
/文献:
/研究领域:
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文献和实验In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
and excision of the lesion and its flanking DNA, and repair DNA synthesis to fill in the resulting single-stranded gap. Here we describe the techniques used to prepare extracts from Saccharomyces cerevisiae cells capable of performing NER reactions
Strategies for Cloning Mammalian DNA Repair Genes
. Only a few human cell-cycle checkpoint genes have been reported, and mammalian genes that correspond closely in function to the RAD6 epistasis group in Saccharomyces cerevisiae are notably lacking. Although two human homologs of RAD6 were identified
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