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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Carbonic anhydrase 2 (CA2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥21000.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2448.0 |
| 规格: | 20μg | 产品价格: | ¥1248.0 |
纯度:
Greater than 95% as determined by SDS-PAGE.基因名:
CA2Uniprot No.:
P00918别名:
Carbonate dehydratase II;Carbonic anhydrase C;CAC;Carbonic anhydrase II;CA-II;Cyanamide hydratase CA2种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Mammalian cell分子量:
30.7 kDa表达区域:
1-260aa氨基酸序列:
MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
Not TestResearch Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验. Balasubramanian, Artificial chaperoning of insulin, human carbonic anhydrase and hen egg lysozyme using linear dextrin chains-a sweet route to the native state of globular proteins, FEBS Lett. 443 (1999) 215-219. 46. M. Yoshimoto, R. Kuboi, Oxidative refolding
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
(DPBS, no Ca2+ no Mg2+ ; Life Technologies, cat. no. 14190250) containing 5% (v/v) fetal bovine serum (FBS) Desired fluorescently labeled anti‐human antibodies and isotype controls (Table
肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响
处的吸光值, 以洗脱液流分体积为横坐标, OD490为纵坐标绘制HSS活性曲线。上述所有样品均以三孔重复测定。 1.5 HSS分子量的鉴定[21]根据1.4结果, 选取HSS活性洗脱部分(相对于220~230 ml流分体积)冷冻浓缩。分别取待测HSS(100 μg)和低分子量蛋白标准(北京天象人生物工程公司)进行SDS-PAGE和硝酸银染色。 1.6 细胞处理 BEL-7402细胞按2×105/ml密度接种于Φ100 mm的培养皿。细胞完全贴壁后, 弃含血清培养基, 以无Ca2+、 Mg
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