CCR6/CCR6蛋白Recombinant Human C

纯度：

The purity information is not available for VLPs proteins.

基因名：

CCR6

Uniprot No.：

P51684

别名：

CKRL3;CMKBR6;GPR29;STRL22

种属：

Homo sapiens (Human)

蛋白长度：

Full Length

来源：

Mammalian cell

分子量：

42.5 kDa

表达区域：

1-374aa

氨基酸序列：

MSGESMNFSDVFDSSEDYFVSVNTSYYSVDSEMLLCSLQEVRQFSRLFVPIAYSLICVFGLLGNILVVITFAFYKKARSMTDVYLLNMAIADILFVLTLPFWAVSHATGAWVFSNATCKLLKGIYAINFNCGMLLLTCISMDRYIAIVQATKSFRLRSRTLPRSKIICLVVWGLSVIISSSTFVFNQKYNTQGSDVCEPKYQTVSEPIRWKLLMLGLELLFGFFIPLMFMIFCYTFIVKTLVQAQNSKRHKAIRVIIAVVLVFLACQIPHNMVLLVTAANLGKMNRSCQSEKLIGYTKTVTEVLAFLHCCLNPVLYAFIGQKFRNYFLKILKDLWCVRRKYKSSGFSCAGRYSENISRQTSETADNDNASSFTM

蛋白标签：

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

货期：

3-7 business days

注意事项：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

功能：

Act as a receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Act as a receptor for non-chemokine ligands such as beta-defensins.

内毒素：

Less than 1.0 EU/ug as determined by LAL method.

SDS-PAGE:

CSB-MP004845HU is detected by Mouse anti-6*His monoclonal antibody. The two bands respectively correspond to monomer, Homodimer.

LC-MS Image Description/Western Blot:

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Product types:

MP-VLP Transmembrane Protein & Active Protein & In Stock Protein

Biological_Activity:

Measured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10 μg/mL can bind Anti-CCR6 recombinant antibody (CSB-RA004845MA1HU). The EC50 is 44.79-56.10 ng/mL. The VLPs (CSB-MP3838) is negative control.

Research Areas:

Cancer

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Reference:

"Identification of CCR6, the specific receptor for a novel lymphocyte- directed CC chemokine LARC." Baba M., Imai T., Nishimura M., Kakizaki M., Takagi S., Hieshima K., Nomiyama H., Yoshie O. J. Biol. Chem. 272:14893-14898 (1997) "Molecular cloning and RNA expression of two new human chemokine receptor- like genes." Zaballos A., Varona R., Gutierrez J., Lind P., Marquez G. Biochem. Biophys. Res. Commun. 227:846-853 (1996) "Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27." Liao F., Lee H.-H., Farber J.M. Genomics 40:175-180 (1997)

Function:

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