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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Acid ceramidase (ASAH1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Acid ceramidase (ASAH1)品名简称:
Recombinant Human ASAH1 protein货号:
CSB-EP619774HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ASAH1Uniprot No.:
Q13510别名:
Acylsphingosine deacylase N-acylsphingosine amidohydrolase Putative 32KDA heart protein Short name:PHP32种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
58.7 kDa表达区域:
22-395aa氨基酸序列:
QHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.功能2:
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.文献:
"A new gene family predicted by a novel human heart cDNA."Churchill J.R., Wieland S.J., Hoffman S., Gallin E.K., Murphy P.M.Mol. Biol. Cell 6:418-418(1995)研究领域:
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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
. The establishment of plasmidsharbouring reporter genes under control of the native promoter sequences provides a tool to validate novel putative Wnt targets by directly quantifying the b-catenin-dependent activation of each specificgene. In this chapter
at one molecule per complex. Plant Cell 1 4 , 689-702. 18. Piotrowski, M., Janowitz, T., and Kneifel, H. (2003) Plant C - N hydrolases and the identification of a plant N-carbamoylputrescine amidohydrolase involved in polyamine biosynthesis. /. Biol. Chem
Studies of the Ubiquitin Proteasome System
Systems Basic Protocol 16: In Vitro Neddylation Labeling and Detection of Ubiquitin Basic Protocol 17: Radiolabeling Ubiquitin with 32P Alternate Protocol 8: Radiolabeling Ubiquitin with 125Iodine Alternate Protocol 9: Labeling Ubiquitin
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