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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Colicin-E7 (colE7)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Colicin-E7 (colE7)品名简称:
Recombinant E.coli colE7 protein货号:
CSB-EP671722ENL规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
colE7Uniprot No.:
Q47112别名:
/种属:
Escherichia coli蛋白长度:
Full Length来源:
E.coli分子量:
68.3 kDa表达区域:
1-576aa氨基酸序列:
MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNPWGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSGEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.; Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.功能2:
/文献:
/研究领域:
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文献和实验相关专题 蛋白表达技术全攻略 影响E.coli 中蛋白表达量的因素除载体 启动子结构以外,还有 质粒拷贝数、质粒稳定性、mRNA结构、密码子的偏爱性和宿主菌的生长状态等因素[58].由于 Vector NTI suitor7.0软件模拟表达,可知mRNA结构和密码子的偏爱性两个影响因素不会造成表达困难,所以本实验的工作主要针对载体拷贝数、载体稳定和宿主菌的生长状态. 本实验中重组 表达质粒PrP-pET-32a(+)不稳
限制性核酸内切酶和修饰酶产生的质粒 携带有编码合成限制性核酸内切酶和修饰酶的基因。 大肠杆菌素质粒 ( Col plasmid ,或称 Col 因子) 携带有产生大肠杆菌素( colicin )酶系基因的质粒,赋予大肠杆菌产生大肠杆菌素的能力。大肠杆菌素( colicin )是一种由 E.coli 的某些菌株所分泌的细菌素,具有通过抑制复制、转录、转译或能量
相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
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