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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bat coronavirus HKU4 Nucleoprotein (N)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3468.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
NUniprot No.:
A3EXA1别名:
Nucleocapsid protein;NC;Protein N种属:
Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)蛋白长度:
Full Length来源:
E.coli分子量:
50.9 kDa表达区域:
1-423aa氨基酸序列:
MATPAAPRTISFADNNDNQPNQQQRGRGRNPKPRPAPNNTVSWYTGLTQHGKNPLAFPPGQGVPLNANSTTAQNAGYWRRQDRKINTGNGVKQLAPRWFFYYTGTGPEANLPFRSVKDGIVWVYEEGATDAPSVFGTRNPANDAAIVCQFAPGTLIPKNFHIEGTGGNSQSSSRASSNSRNSSRSSSRGGRSTSNSRGTSPVSHGVGSAESLAALPLLLDLQKRLADLESGKSKQPKVVTKKDAAAAKNKMRHKRVATKGFNVTQAFGLRGPGPLQGNFGDMNYNKFGTEDPRWPQMAELAPSASAFMSMSQFKLTHQSNDDKGDPIYFLSYSGAIKLDPKNPNYKKWLELLESNIDAYKTFPKKERKPKTTEDGAVASSSASQMEDVDAKPQRKPKSRVAGSITMRSGSLPALQDVTFDSEA蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
5位院士为代表,超12篇CNS大作,5月新冠研究汇总——全方位围攻新冠病毒,值得收藏
病毒所石正丽团队与福建师范大学欧阳松应教授在 bioRxiv 上发表题为Evolutionary arms race between virus and host drives genetic diversity in bat SARS related coronavirus spike genes 的研究。结果表明,SARSr-CoV Spike蛋白和中华菊头蝠 ACE2 可能是随着时间的推移共同进化的,并经历来自彼此的选择压力,引发军备竞赛(Arms race)的协同进化关系,进一步证明中华菊
Overview of Protein Microarrays
., Han, Y., Zhang, Y., Song, Y., Yao, X., Tao, Y., Zeng, H., Yang, H., Wang, J., Zhu, H., Chen, Z.‐N., and Wu, L. 2007. A protein chip approach for high‐throughput antigen identification and characterization. Proteomics 7:2151‐2161
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