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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
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- 保质期：
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- 英文名：
Azido-PEG7-Alcohol
- 库存：
10
- 供应商：
南京北鱼生物科技有限公司
- CAS号：
1274892-60-2
- 规格：
Kg
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文献和实验with organic extraction. Finally, the DNA is purified by precipitation with alcohol and visualized by gel electrophoresis.Protocol: 1. Add RNase A and DNase I, each to a final concentration of 1mg/mL, into 10 mL of plate lysate of phage in a 30 ml Corex tube
the pellet by repeated pipetting. This may take some work. Transfer the suspended phage to a microfuge tube. 11. Extract twice with 1 volume of chloroform (containing no isoamyl alcohol!). This step removes some of the PEG while the phage are still intact
with 1 volume of chloroform (containing no isoamyl alcohol!). This step removes some of the PEG while the phage are still intact. 12.Add an equal volume of phenol to burst phage and to begin removing phage proteins. Mix by inversion and spin in a microfuge for about 1-3min
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