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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 适应物种:
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
- 库存:
99
- 宿主:
Mouse
- 应用范围:
ELISA,IF,WB
- 浓度:
见说明
- 抗体英文名:
Anti-Influenza A virus M2 ectodomain/M2e Monoclonal antibody (Fab65)
- 规格:
50-100ug
小鼠抗Influenza A virus M2 ectodomain/M2e单克隆抗体(克隆号Fab65)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验严重的是容易造成探针凝聚,并严重影响标记活性。因为探针溶液中的游离蛋白容易抢先与标记位点结合,起到“封闭”(Blocking)作用,而胶体金探针标记不上。在标记位点希少、被标记物含量较少的情况下要特别注意。 这里需要特别指出的是,使用不同直径的胶体金与同一蛋白结合时,除了蛋白量完全不同外,往往最佳pH也有一定变化。 因此,在探针制备每一环节应随时监测探针对分布情况、负染结合及活性。 1、抗血清的前处理 一般抗血清中IgG的含量为10-25%,而绝大部分为其它蛋白。用抗血清直接制备探针其标记活性
基因以外的,不是由细胞DNA中的永久性变化引起的细胞中稳定的变化。在细胞发育过程中细胞的分化大多反映基因以外的变化。 Epitope 表位,被特异抗体或T细胞识别的抗原分了上的一个靶区。一人抗原分子可含有多个针对不同单克隆抗体的不同表位。 Epstein-Barr virus(EBV) 具有DNA型遗传信号的γ疱疹病毒。其原始宿主是人。这种几种到处存在的病毒主要感染B细胞并在缺乏免疫监视时使它们转化。它与传染性单核细胞增多症、鼻咽癌及多种
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